Venn diagram of 3-way unfused suture comparison results. Global expression differences were compared for 3 pair-wise comparisons (coronal vs sagittal, lambdoid vs coronal, lambdoid vs sagittal). The sagittal suture showed the greatest difference in gene expression to the other two sutures, followed by the lambdoid suture. A) Increased genes. 62 genes had similar increased expression in coronal and lambdoid sutures compared to sagittal sutures and 33 genes had increased expression in lambdoid sutures and similar expression in coronal and sagittal sutures. B) Decreased genes. 188 genes had similar decreased expression in unfused coronal and lambdoid sutures compared to unfused sagittal sutures and 2 genes had decreased expression in lambdoid sutures and similar expression in coronal and sagittal sutures.