Figure 3

RT-PCR analysis of RNA samples used and validation of microarray results. Ratio, ratio of differentiated (Day 16)/undifferentiated (Day 0) hESC sample normalized spot intensity as detected by microarray analysis (average value from all spots). M, 100 bp DNA ladder; Day 0, undifferentiated hESCs; Day 16, hESCs committed to neuronal/dopaminergic differentiation pathway by co-culturing with PA6 stromal cells for 16 days; C-, No template control. Sox2, SRY-box 2; En1, Engrailed 1; Gapdh, glyceraldehydes-3-phosphate dehydrogenase; Aldh1a1, aldehyde dehydrogenase 1 family, member A1; Sdha, succinate dehydrogenase 2, flavoprotein sububit; Tubb, β-tubulin; Actb, β-actin; Th, tyrosine hydroxylase; Msx1, homolog of Drosophila muscle segment homolog 1; Pitx2, paired-like homeodomain transcription factor 2.