Figure 4
NeuroStem Chip layout (a fragment). (A) Representative block (31 × 31 spots), as hybridized with actual hESC (Cy3)/universal reference (Cy5) sample. Arrow indicates β-actin (Actb) spot, which serves as a control for grid orientation; white boxes (B) highlight repetitive patterns, illustrating quadriplication of individual spots. (B, C) Dye-swap (hESC (Cy5)/universal reference (Cy3)) indicates accuracy of fluorescent dye incorporation and hybridization chemistry. Ier5, Immediate early response 5; Rab35, RAS-associated protein RAB35; Zbtb7, Zink finger and BTB domain containing 7. Spot-to-spot center distance 140 μm; average spot size 90–110 μm.