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Figure 5 | BMC Genomics

Figure 5

From: "NeuroStem Chip": a novel highly specialized tool to study neural differentiation pathways in human stem cells

Figure 5

Normalization and reproducibility of the NeuroStem Chip arrays. (A-B) Representative plots depict Log2(ratio) versus log10(intensities) prior to (A) and following (B) the normalization of one technical replicate (hESCs (Cy3) : universal reference (Cy5), 10:5 pmol). Green lines represent fitted values after normalization. (C) Centroid graph of a K-means classifier of 101 genes clustered as highly up-regulated in the 4 technical replicates, indicating high reproducibility. Pink line is an average of the Log2 ratio values of the 101 genes. See [Additional file 1] for a list of genes in this cluster.

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