Homologous sequences for Ar. subalbatus found in fly databases. A) Comparative analysis of Ar. subalbatu s EST clusters with predicted peptides from 3 other mosquito species with completed genomes: Ae. aegypt i, An. gambiae, and C.p. quinquefasciatus. Overlapping regions indicate homologous sequences from blastx searches against the peptide databases. A homolog is defined as having an e-value cutoff of 1e-20, a percent match of 40% (true matches, not conserved), and a minimum match length of 30 for the high-scoring segment pair (HSP). This comparison includes 8,020 possible cluster sequences from Ar. subalbatus (brackets), of which 3,013 had no homolog. Boxes directly adjacent to circles indicate 1) the species being compared to Ar. subalbatus, and 2) the total # of homologous sequences between that species and Ar. subalbatus. B) A gene list of the total of overlapping and non-overlapping Ar. subalbatus homologs to Ae. aegypt i, An. gambiae, and C. p. quinquefasciatus was compared to a gene list of homologs found to D. melanogaster. A significant number of genes (2,074) from Ar. subalbatus have no homolog in the fruit fly, but qualify as homologs to genes in other mosquito species.