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Table 4 Searching Ar. subalbatus EST clusters for immunity-related sequences based on homology to other flies.

From: Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus

Family Subfamily asuhem diroinf imacbac brumal naïve7 naïve14 Total Reads R Value
AMP Cecropin 98 65 70 56 41 47 377 0.070988
AMP Defensin 238 41 78 16 15 28 416 0
AMP Diptericin 0 2 0 0 0 1 3 0.160764
CASP CASP 1 1 0 1 1 0 4 0.673041
CAT CAT 0 1 0 0 0 0 1 0.561495
CLIP CLIP-CLIP-CLIP-SPH 1 1 1 0 0 1 4 0.670142
CLIP CLIP-SP 136 33 37 24 22 27 279 0
CLIP CLIP-SP-CLIP-SP 1 0 0 1 0 0 2 0.668311
CLIP CLIP-SPH 153 33 55 58 14 47 360 0
CLIP SP-CLIP 4 0 0 0 0 0 4 0.060745
CLIP SP-CLIP-SPH 2 0 0 1 0 0 3 0.440816
CLIP SPH-CLIP 1 0 0 0 0 0 1 0.754901
CLIP SPH-CLIP-SPH 1 1 0 2 0 1 5 0.576541
CLIP SP-SPH-CLIP 0 1 0 0 0 0 1 0.561495
CTL CTL 80 19 27 17 25 17 185 0.000002
FREP FREP 17 9 22 4 4 8 64 0.000764
GALE GALE 0 0 2 1 1 2 6 0.206431
GNBP GNBP 0 1 1 0 0 0 2 0.420516
IAP IAP 1 1 0 3 0 2 7 0.260929
IMDPATH IKKg 1 0 0 1 0 0 2 0.668311
LYS LYSC 122 21 37 19 15 42 256 0
ML ML 14 10 5 8 9 3 49 0.223609
PGRP PGRP 9 1 4 2 2 5 23 0.312906
PPO PPO 1 1 0 1 0 0 3 0.653363
PRDX GPX 8 2 1 1 0 3 15 0.100553
PRDX HPX 1 2 1 3 1 0 8 0.446023
PRDX TPX 7 3 5 14 4 8 41 0.136651
SCR SCRA 0 7 3 1 2 2 15 0.004735
SCR SCRB 1 0 0 1 2 0 4 0.26927
SCR SCRC 1 0 0 0 0 0 1 0.754901
SOD SOD-Cu-Zn 5 3 3 7 2 2 22 0.798952
SOD SOD-Mn-Fe 30 5 4 2 4 6 51 0.000052
SPZ SPZ 0 0 1 0 0 0 1 0.575875
SRPN SRPN-INHIB 26 8 8 2 8 4 56 0.001248
SRPN SRPN-nonINHIB 4 2 2 0 3 2 13 0.281764
TEP TEP 1 1 2 1 1 4 10 0.376665
TOLLPATH TUBE 0 0 0 0 0 1 1 0.562898
  Totals 965 275 369 247 176 263 2295  
  1. Peptide sequences were harvested from ImmunoDB [24] for An. gambiae, Ae. aegypti, and D. melanogaster for each of the following gene families: All 8,020 EST clusters were blasted against a database of these peptides using blastx (e-value cutoff of 1e-5). BLAST hits were parsed from output files using tcl_blast_parser_123_V017.tcl [55] with a cut-off of 40% match, 1e-20 normalized e-value, and a minimum match length of 30 residues. Top hits were taken for each significant match and verified manually. Matches then were categorized by family and subfamily according to Waterhouse et al. (2007) (Left column) and the composition (# of compiled sequences) of EST clusters were collated from the assembly according to the library(s) from which ESTs came: asuhem (bacteria-inoculated, hemocyte), diroinf (whole body, Dirofilaria immitis injected), imacbac (bacteria-inoculated whole body), brumal (blood-fed Brugia malayi), n7 and n14 (naïve, 5–7 and 12–14 days of age). Stekel R values [23] were determined for the groupings using IDEG6 [20] and those having a score of 0.001 or better were considered significantly differential, and are flagged with bolded and underlined text for the row.