A BAC pooling strategy combined with PCR-based screenings in a large, highly repetitive genome enables integration of the maize genetic and physical maps

Table 1 SSR markers with a low recombination rate on the IBM genetic map, using the physical map to redefine their order

Bin	Map coordinate	SSR markers	GenBank Accession #	Contigs hit (#of BACs hit within contig)
1.05	467.0*	p-umc1906	B68068	33(3)
	467.9	p-umc1903	G68065	33 (6)
3.04	190.6	p-bnlg1019	-	116(5)
	190.8	p-umc1717	AW573521	117 (1)
	190.8*	p-bnlg1452	-	116 (2)
	190.8*	p-bnlg1113	-	117 (4)
	191.1	p-umc1655	AZ916071	116 (5)
5.06	492.6	p-umc1941	-	250(4)
	493.5*	p-umc1680	AW261378	250 (1)
	493.5*	p-umc1524	AQ844589	249 (2)
	493.7	p-phi087	-	250 (4)
	500.1*	p-umc2306	BI431083	250 (4)
	500.7*	p-bnlg609	-	250 (7)
6.04	181.9*	p-umc65	G10860	276 (2)
	189.5*	p-umc1796	AW562820	b0218P06^a
	189.9	p-umc1918	G68080	276 (5)
7.03	405.5*	p-umc1301	AI947719	322 (7)
	405.5*	p-umc1936	G68098	322 (2)

^ap-umc1796 identified a single BAC clone, b0218P06. This clone was not fingerprinted and thus not displayed in WebFPC data. * on-frame position on the map.

ISSN: 1471-2164