Microarray analysis of iron deficiency chlorosis in near-isogenic soybean lines

BMC Genomics

Table 2 Genes differentially expressed between near-isogenic lines that did not cluster with other iron or stress induced genes.

Clone ID	Federated Ratio	P-Value	Associated TIGR TC	UniProt	TBLASTX UniProt DB Annotation	UniProt Blast E-Value
Gm-c1028-8390	2.217	0.1037	BE021708	Q7XZ14	Transcription Factor DP1	7.00E-11
Gm-c1028-6580	2.247	0.0403	GmTC215393	Q06364	26S Proteasome non-ATPase Regulatory Subunit	1.00E-177
Gm-c1028-4867	2.379	0.0316	AW831377	Q8RWY1	2OG-Fe(II) Oxygenase	1.00E-25
Gm-c1028-7485	2.471	0.0311	GmTC219105	Q940G0	Endomembrane Protein	1.00E-133
Gm-c1028-2190	2.471	0.0426	GmTC227948	Q6DBF6	Membrane Protein	3.00E-35
Gm-c1028-720	2.663	0.0065	GmTC227091	Q949M9	Putative arsA Homolog hASNA-1	1.00E-147
Gm-c1004-5020	2.772	0.0241	GmTC225133	Q8JUF1	Large Polyprotein 2	0
Gm-c1004-6717	2.788	0.1011	GmTC203969	Q7XYW5	Plant Specific Membrane Protein	3.00E-18
Gm-c1009-2578	2.892	0.0551	AW278268	No UniRef	No UniRef Hit E < 10^-4	NA
Gm-c1028-8336	3.087	0.0004	BE021665	No UniRef	No UniRef Hit E < 10^-4	NA
Gm-c1004-6231	3.429	0.0599	GmTC204328	Q3HVN0	Ubiquitin Conjugating Enzyme	5.00E-59
Gm-c1013-2943	3.435	0.1685	GmTC226909	Q9C9T6	Zinc Ring Finger Protein	8.00E-61
Gm-c1028-1850	3.532	0.0179	GmTC229698	Motif Analysis	TIR-NBS-LRR-TIR Type Disease Resistance Protein	NA
Gm-c1028-4530	3.532	0.0102	AW704680	Q1SL19	Nonsense Mediated Decay Protein UPF3	3.00E-51
Gm-c1028-8658	3.564	0.0041	BE021924	Q6W5B6	Ethylene Receptor	4.00E-11
Gm-c1028-8183	3.687	0.2337	BE021484	Q9LR39	No UniRef Hit E < 10E-4	NA
Gm-c1028-3740	3.712	0.0359	GmTC217970	Q9LY38	Phagocytosis and Cell Motility Protein	3.00E-32
Gm-c1028-1088	4.181	0.1264	GmTC217285	Q8H9B4	UDP-glucosyltransferase	1.00E-179
Gm-c1028-963	7.149	0.0057	GmTC225698	Q2TE73	Zinc Ring Finger Protein	1.00E-108

This list of genes represents the genes identified as differentially expressed between the NILs under iron deficient conditions which do not have sequence homology to other known stress induced genes. The sequences of these genes also showed no homology to other genes induced by iron deficiency and differentially expressed between the NILs. The clone ID identifies the specific clone spotted on the microarray. The Federated Ratio is the fold change between the two near isogenic lines. Fold changes above 2 represent genes over-expressed in the iron inefficient plant compared to the iron efficient plant while fold changes below 0.5 represent genes under-expressed in the iron inefficient plant compared to the iron efficient plant. The TIGR TC represents the tentative consensus sequence to which the clone ID belongs according to TIGR. The UNIPROT annotation is the identified function of genes showing high similarity to the sequence of the TIGR TCThe E-value is the association of the annotation to the TIGR TC sequence.

ISSN: 1471-2164