Simple sequence repeats in zebra finch (Taeniopygia guttata) expressed sequence tags: a new resource for evolutionary genetic studies of passerines

Table 4 Amplification success of ten EST-SSR markers in two zebra finch and two house sparrow populations.

GI number	Primers	Expected size	Zebra finch					House sparrow
			Size range	Sheffield		Australia		Size range	Lundy		Aldra
				k	H_o	k	H_o		k	H_o	k	H_o
CK304956	F-GGAATGCGTGGACCTTCTGGT R-TAGATTCTTTCATCAAGTTTCTGCCC	184	184–187	3	0.444	Not tested	Not Tested	181–185	4	0.60	4	0.76
DV952809 (contig23)	F-CACATCAAAGGATGTGGTGG R-CAATGTGAATTGCAGGGTCA	192	189–191	2	0.25	2	0.38	195–197	2	0.44	1	0.00
DV952125	F-TGGATTACCTGTCTGAAAGACC R-TTCACTGTCTAGTCCAACCCTGT	206	202–210	5	0.86	5	0.5	151–171	2	0.28	2	0.43
DV955012	F-TCTCAGCATCAGTGTGTTCTGTAC R-TTTGCTTGGGATTGAAGTGA	289	282–307	6	0.91	3	0.52	296–298	2	0.35	1	0.00
CK313552	F-GGAGTTGGAGCGCAAGAAGC R-CGTCTTCCAAATCCGTGAGT	235	-	-	-	-	-	-	-	-	-	-
DV946651	F-CACAGAAAAGTGAGTGCATTCC R-TGGGAAAACATCTTTACCATCA	249	243–262	3	0.35	5	0.89	287–308	5	0.64	5	0.60
CK307697 (contig35)	F-CGAGTAGCGTATTTGTAGCGA R-AGGAGCGGTGATTGTTCAGT	180	170–183	6	0.79	4	0.62	171–181	6	0.55	4	0.66
DV948303	F-CTTCCTGCTATAAGGCACAGG R-AAGTGATCACATTTATTTGAATAT	118	107–117	3	0.38	6	0.74	96–105	3	0.50	-	-
CK312585 (contig 73)	F-ATTCTCAGGTCAATCGCTCACACT R-CAATGCAACAATCTACTCAACACC	434	-	-	-	-	-	-	-	-	-	-
DV959049 (contig 206)	F-GGGGACGTTTTATCTGTTACC R-GCAGTGCCCAAGTTCAGAGT	244	207–230	4	0.47	9	0.59	-	-	-	-	-

GI number is the Genbank identification number of each EST sequence. k = number of alleles, H_o = observed heterozygosity. 24 birds were typed from each population. All were in Hardy-Weinberg Equilibrium. All are dinucleotides except CK313552 which is a tetranucleotide. Further details on these EST-SSRs (including repeat motif and length, and predicted chromosomal assignments) are available in Additional Files 1 and 2.

ISSN: 1471-2164