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Figure 4 | BMC Genomics

Figure 4

From: An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*

Figure 4

Two-dimensional gel electrophoresis of 50 μg salivary protein from adult female Ædes ægypti mosquitoes. Numbers on the left indicate molecular weight marker positions in the gel. The + and - signs indicate the anode or cathode side of the isoelectrophocusing dimension, which ranged from pH3-10. Gel bands that were identified to a protein (following tryptic digestion and mass spectrometry) are shown in the gel. In some cases, more than one band accounted for the same protein, possibly due to trailing or multiple isoforms. The proteins found were: D7l1 (gi: 16225992), PNase (purine nucleosidase, gi: 21654712) PDI (protein disulfide isomerase, gi: 94468800), HS70 (heat-shock protein 70 KDa, gi: 94468818), F0F1β (F0F1 ATPase beta subunit, gi: 94468834), Ang1 (angiopoietin-like protein, gi: 18568298), Serp1 (salivary serpin1, gi: 18568304), 62 k (62-kDa proteins gi: 18568300 and gi: 18568302), Serp2 (salivary serpin2, gi: 94469320), amylase (gi: 2190949), ADA (adenosine deaminase, gi: 18568326), apyrase (gi: 1703351), F0F1α (F0F1 ATPase, alpha subunit, gi: 94468442), D7l2 (gi: 118216), Ag5 (antigen5 protein, gi: 18568284), C-Lec2 (C-type lectin, gi: 94468370), 34k1 (34-kDa protein 1, gi: 94468642), Ang2 (angiopoietin-like protein2, gi: 94468352), 34 k2 (34-kDa protein2, gi: 18568296), C-Lec1 (C-type lectin 1, gi: 18568318), actin (gi: 94468486), 30.5 (30.5-kDa salivary protein, gi: 61742033), and 30ag (30-kDa antigen, gi: 18568322). For experimental details, see Methods.

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