An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*

Ribeiro, José MC; Arcà, Bruno; Lombardo, Fabrizio; Calvo, Eric; Van My Phan; Chandra, Prafulla K; Wikel, Stephen K

doi:10.1186/1471-2164-8-6

BMC Genomics

Table 2 Classification of 71 selected genes encoding putative secretory products as determined by RT-PCR expression analysis on female salivary glands, whole male mosquitoes, and carcass of female mosquitoes

From: An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*

Protein name or type	Comments	SG	ENR	SG, M	Ubiq	#
Secreted carrier-like proteins including D7 family
gi\|61742027	D7s1	X
gi\|94468624	D7s2 allele	X
gi\|18568330	D7s3	X				#
gi\|16225992	D7l1			X
gi\|118216	D7l2		X
gi\|94468424	D7s4	X
Secreted protease inhibitors
Serpins
gi\|94468342	Similar to FXa-directed anticlotting	X				#
gi\|94469320	Salivary serpin	X
gi\|18568304	Serpin			X		#
Other protease inhibitors
gi\|94468720	Kazal-containing peptide				X
gi\|94468612	Cystatin				X
Enzymes
Nucleotidases
gi\|94469274	Second apyrase or 5' nucleotidase			X
gi\|21654712	Salivary purine nucleosidase			X
gi\|1703351	Salivary apyrase			X
gi\|18568326	Putative adenosine deaminase		X
Serine proteases
gi\|94468658	Serine protease			X		#
gi\| 94468410	Salivary chymotrypsin-like enzyme			X
gi\|18568306	Putative serine protease			X
gi\|94468372	Trypsin like			X
Immunity-related proteins
Lectins
gi\|94468370	Putative salivary C-type lectin	X
gi\|18568318	Putative C-type lectin	X				#
gi\|94468698	C type lectin				X
gi\|94468694	C-type lectin				X
Angiopoietin
gi\|18568298	Angiopoietin-like protein	X				#
gi\|94468352	Angiopoietin-like protein splice variant	X
Antimicrobial polypeptides
gi\|48256697	Defensin A1				X
gi\|18568310	Gambicin				X
gi\|18568288	Putative lysozyme			X
gi\|94468690	Putative salivary peptide with HHH domain			X
Other
gi\|94468654	i23M allele				X
gi\|18568294	Gram-negative binding protein			X
gi\|94468610	Peptidoglycan recognition protein				X
Unknown function, secreted, and ubiquitous
Antigen 5 protein family
gi\|18568284	Antigen 5 member	X
gi\|18568278	Putative secreted protein			X
gi\|18568308	Antigen 5 family member			X
Other secreted proteins found in non-bloodsucking insects
gi\|94468620	PAN/APPLE-like domains				X
gi\|94468538	Cysteine-rich venom-like protein			X		#
Unknown function, found in hematophagous Diptera salivary transcriptomes
56.5 kDa
gi\|18568292	Putative 56.5 kDa secreted protein			X
41-kDa mosquito family
gi\|94468350	Putative 41-kDa salivary secreted protein				X	#
30-kDa/GE-rich family
gi\|94468546	30-kDa salivary gland allergen variant 2	X				#
gi\|18568322	Putative 30-kDa allergen-like protein	X				#
29-kDa family
gi\|94468416	Putative salivary protein		X
Other salivary proteins found in hematophagous Diptera
gi\|94468392	Putative secreted protein				X
gi\|94468374	Similar to An. gambiæ gSG8 protein			X
gi\|61742023	Putative 8.9-kDa secreted protein			X
gi\|94468386	Putative 23.4-kDa salivary protein			X
gi\|94468986	Putative secreted peptide				X
gi\|94468460	Putative secreted peptide				X
gi\|94468614	Unknown secreted	X
gi\|94468634	Possible secreted protein	X				#
gi\|94468408	Putative secreted salivary peptide				X
Aedes genus specific
62kDa family – Single exon
gi\|18568300	Putative secreted protein	X				#
gi\|18568302	Putative secreted protein	X				#
34-kDa gene family
gi\|94468412	Salivary peptide similar to 34-kDa protein		X
gi\|94468650	Putative salivary peptide	X
gi\| 94468336	Putative 34-kDa secreted protein family	X
gi\|18568296	Putative 34-kDa secreted protein		X
30.5-kDa
gi\|94468432	Putative 30.5-kDa secreted protein		X
gi\|61742033	Putative 30-kDa secreted protein	X				#
9-kDa family
gi\|94468426	Salivary peptide with WWW domain			X
gi\|94468396	Putative secreted peptide			X
Other Aedes-specific polypeptides
gi\|94468530	Putative salivary secreted peptide				X
gi\|94468356	Putative salivary peptide with basic tail		X
gi\|18568314	Putative 18.6-kDa secreted protein			X
gi\|94468380	Putative 18.6-kDa secreted protein – variant			X
gi\|18568282	Putative 7.8-kDa secreted protein		X			#
gi\|94468390	Putative basic peptide 4.2K-1	X
gi\|94468394	Proline-rich peptide	X
gi\|94468568	Putative salivary secreted peptide			X
gi\|42632615	Putative 8.7-kDa secreted protein	X				#
gi\|94468646	Peptide similar to insulin-like growth factor				X

The four classes are SG (signal found exclusively in adult female salivary glands); ENR (enriched in adult female salivary glands); SG,M (found in adult female salivary glands and in males, but not in carcasses of adult females lacking salivary glands); and Ubiq (genes ubiquitously found in all tissues tested). Clones marked with # were analyzed three times, all others twice. Clones 94468370, 48256697, and 94468634 may undergo alternative splicing. Although clones 94468546 and 18568322 (30 kDa), 94468614, 18568300, and 18568302 (62 kDa) were assigned to SG class, some faint bands were seen in some samples from males. Clones 94468620, 94468350, 94468634, and 42632615 (primer pairs 23, 3, 29, and 60) underwent 35 cycles due to low amplification levels.

Back to article page

ISSN: 1471-2164

Contact us

Submission enquiries: bmcgenomics@biomedcentral.com
General enquiries: ORSupport@springernature.com