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Table 5 Validation of differential expression by real time PCR.

From: Target genes of myostatin loss-of-function in muscles of late bovine fetuses

Gene

Ratio NM/DM by array analysis

Ratio NM/DM by RT-PCR

TPM3

3.38*

4.45*

ATP2A2

2*

2.85*

MYH7

4.86-*3.37*

3.28*

C1QTNF3

1.87*

2.97*

PLN

5.48*

4.31*

COL1A

5.22*-2.98*

10

BGN

1.79*

5.05*

PLOD3

1.80*

1.4*

  1. Results are means of duplicate experiments. *All differential expressions are statistically significant at P < 5% for array experiments (using SAM analysis; comparison of 2 NM vs 2DM animals) or by real-time RT-PCR (using the Mann Whitney U Test).