Table 1 PCR primers used in study.

Probe set

Primer name

Direction

AT

Primer sequence (5' – 3')

Target region

115.m00143_at

115.m00143F

S

55°C

CCAAACGATACACACCCAGA

Coding

115.m00143R

AS

55°C

GCAATTTGAAACTTCACATCACA

Coding

141.m00082_at

141.m00082F

S

55°C

TCATTTGGAATTGTTTATGTTGG

Coding

141.m00082R

AS

55°C

TGCACTTCCAAAATCCGTTA

Coding

2.m00545_at

2.m00545F

S

55°C

GCTGCCATGACTAATGCTGA

Coding

2.m00545R

AS

55°C

AGCAACAGCAACTGGTCCTT

Coding

226.m00092_at

226.m00092F

S

55°C

GCAAACATGGGATACAGCAG

Coding

226.m00092R

AS

55°C

ACAAGGCGCTTGTTCAACTT

Coding

(B) Primers used in PCR from bisulfite treated DNA

Probe set

Primer name

Direction

AT

Primer sequence (5' – 3')

Target region

64.m00187_s_at

EHsp5'bis*

S

50°C

ATGAATAAGAAAGTGTGAATAATAG

Promoter

EHsp3'bis*

AS

50°C

AACATTAATTCCACTATTTCCTACTA

Promoter

EHsp1005'*

S

50°C

TGAGTATTTAAAGGAACTTGAAG

Coding

EHsp3'bis*

AS

50°C

AACATTAATTCCACTATTTCCTACTA

Coding

115.m00143_at

115.mPF

S

52°C

CTTGAAGGATAAAAAATAAGTCATATTTCTA

Promoter

115.mPR

AS

52°C

ATAATCTGCATGGCATATATTTCCTAATT

Promoter

115.m00143_at

115.mBCF

S

48°C

ATGTTTGTATCTTATTTTCTATTTTTAATTT

Coding

115.mCR

AS

48°C

GTTCCAGTACATACAATTTTACCT

Coding

141.m00082_at

141.mBPF

S

52°C

TATTGAACACTGCTCTAAATCCACT

Promoter

141.mBPR

AS

52°C

GTGTAGTACTGAATAAACACTTTTCTT

Promoter

141.mBCF

S

53°C

TTGATATAACTAATTCACCTACATTCCAA

Coding

141.mBCR

AS

53°C

GATCCTTCTCCTATTTTCTTTTCTTCT

Coding

97.m00140_at

97.mPF

S

50°C

ATTACTCCTTCCCTCTCTTCTT

Promoter

97.mPR

AS

50°C

CCATTRTCATTTCTCTCAACC

Promoter

194.m00103_at

194.mPF

S

50°C

GACTRCACCCAATTTTCCACCT

Promoter

194.mPR

AS

50°C

CCATCCCAATAAATTCCTTCTT

Promoter

687.m00016_at

687.mBPF

S

53°C

CTTTCARACACTTAACAAATCTTCTTCA

Promoter

687.mBPR

AS

53°C

ARATTCTCTCAAATACACTTCCCAC

Promoter

3.m00674_at

3.mBPF

S

49°C

GTTATTCAATTATACTTTATACCAAACA

Promoter

3.mBPR

AS

49°C

TCATTTTARTTCTTTTTTCTTATACCACT

Promoter

160.m00098_at

160.mBPF

S

50°C

CTAATAAAATTTTCTTACACCTAACTCA

Promoter

160.mBPR

AS

50°C

CTTCTTTATTTTTTCCATAATATTCTTTCT

Promoter

26.m00304_at

26.mBPF

S

48°C

TAAAACTARACCATTTATTCAACAATT

Promoter

26.mBPR

AS

48°C

RRTTCTTCTTCCATAATTTATTATTA

Promoter

2.m00545_at

2.mBPF

S

50°C

TTACTTCTCGTTATTCTATTAATATAAAC

Promoter

2.mBPR

AS

50°C

GATTATTTTTTGCCATCCATTTATCAA

Promoter

2.mBCF

S

50°C

CTAAATTTACAAAAAAACTAATATACTCTC

Coding

2.mBCR

AS

50°C

TAGAGCCACCATTACATCCATTA

Coding

226.m00092_at

226.mBPF

S

49°C

CTATTGATATTGAAATGCAAAAAACAAT

Promoter

226.mBPR

AS

49°C

TGATATATCAACTTAAACAAACTTACTA

Promoter

226.mBCF

S

50°C

GTTCTTCTAGATTATTAAAAACCATTC

Coding

226.mBCR

AS

50°C

AACACTTCCTTTATCTATTTTATTTCC

Coding