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Table 2 Genes up-regulated in 5-AzaC treated E. histolytica HM-1:IMSS parasites as compared to untreated controls.

From: Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression

Probe set Baseline expression level Fold-change p-value Annotation
763.m00027_s_at a 0.01 28.97 0.024 hypothetical protein
120.m00098_x_at 0.01 14.35 0.011 hypothetical protein
329.m00054_at 0.01 13.06 0.017 hypothetical protein
253.m00081_x_at 0.02 10.61 0.041 hypothetical protein
16.m00301_at 0.01 9.75 0.006 hypothetical protein
228.m00060_x_at 0.02 9.43 0.007 AIG1 family protein, putative (GO:0004765)
244.m00069_x_at 0.01 9.23 0.021 hypothetical protein
371.m00031_x_at 0.01 8.69 0.006 BspA-like leucine rich repeat protein, putative
295.m00030_at 0.02 8.11 0.036 conserved hypothetical protein
64.m00173_x_at 0.01 7.95 0.043 BspA-like leucine rich repeat protein, putative
94.m00138_at 0.02 7.09 0.033 hypothetical protein
36.m00220_s_atb 0.02 5.78 0.005 hypothetical protein
432.m00029_x_at 0.08 5.32 0.001 conserved hypothetical protein
1087.m00005_at 0.03 5.12 0.038 hypothetical protein
401.m00029_at 1.11 4.97 0.022 protein kinase, putative (GO:0005524)
92.m00177_x_at 0.05 4.39 0.009 hypothetical protein
86.m00156_s_atc 0.09 4.06 0.036 glutamine cyclotransferase, putative
287.m00045_x_at 0.03 3.93 0.026 hypothetical protein
450.m00030_at 0.12 3.90 0.043 hypothetical protein
2.m00587_x_at 0.04 3.83 0.009 hypothetical protein
115.m00143_at* 0.12 3.64 0.001 transcription initiation factor TFIID, putative (GO:0003677)
420.m00021_x_at 0.03 3.59 0.007 hypothetical protein
27.m00245_at 0.03 3.55 0.017 hypothetical protein
103.m00185_at 6.55 3.32 0.023 Fe-hydrogenase, putative (GO:0005489)
194.m00103_at* 0.07 3.27 0.029 protein kinase, putative (GO:0005524)
26.m00274_x_at 0.07 3.22 0.039 hypothetical protein
935.m00010_at 0.11 3.15 0.009 hypothetical protein
93.m00158_s_at d 0.10 3.10 0.014 DNA mismatch repair protein mutS, putative (GO:0006298)
97.m00140_at* 0.08 3.10 0.006 hypothetical protein
138.m00086_x_at 4.75 3.09 0.023 hypothetical protein
66.m00163_s_at 0.03 3.08 0.010 hypothetical protein
12.m00286_at 0.04 3.06 0.025 hypothetical protein
295.m00044_x_at 0.07 3.04 0.017 conserved hypothetical protein
141.m00082_at* 0.32 2.98 0.010 protein kinase, putative (GO:0004672)
267.m00069_at 0.04 2.96 0.001 hypothetical protein
4.m00648_x_at 0.04 2.89 0.032 hypothetical protein
112.m00119_at 0.04 2.85 0.035 hypothetical protein
472.m00058_s_ate 0.08 2.83 0.008 hypothetical protein
204.m00098_x_at 0.22 2.77 0.008 hypothetical protein
583.m00011_at 0.41 2.69 0.026 hypothetical protein
62.m00174_x_at 0.06 2.66 0.037 hypothetical protein
55.m00164_at 0.07 2.63 0.002 hypothetical protein
273.m00075_x_at 0.05 2.59 0.042 hypothetical protein
249.m00077_x_at 0.06 2.55 0.049 hypothetical protein
289.m00092_at 0.05 2.53 0.014 hypothetical protein
464.m00030_at 0.05 2.53 0.010 hypothetical protein
1.m00665_at 1.11 2.53 0.046 hypothetical protein
51.m00150_x_at 0.05 2.49 0.009 hypothetical protein
159.m00103_x_at 0.43 2.44 0.044 protein kinase, putative (GO:0004672)
19.m00288_at 0.07 2.36 0.005 hypothetical protein
308.m00054_x_at 0.05 2.34 0.009 hypothetical protein
5.m00447_x_at 0.12 2.33 0.016 hypothetical protein
51.m00167_at 0.05 2.33 0.042 hypothetical protein
27.m00266_x_at 0.06 2.30 0.050 ELL complex EAP30 subunit, putative
222.m00072_at 0.09 2.30 0.040 SPRY domain protein
183.m00091_x_at 8.10 2.30 0.026 acyl-CoA synthetase, putative (GO:0008152)
289.m00067_s_atf 0.09 2.30 0.004 short chain dehydrogenase family protein
43.m00197_x_at 7.97 2.24 0.024 cell adhesion protein (GO:0007155)
54.m00234_s_atg 1.09 2.22 0.037 hypothetical protein (GO:0005554)
4.m00613_x_at 0.12 2.21 0.007 hypothetical protein
48.m00224_s_ath 0.71 2.21 0.018 acetyltransferase, putative
75.m00156_x_at 0.11 2.17 0.007 myotubularin, putative
25.m00258_x_at 0.38 2.16 0.032 hypothetical protein
34.m00243_x_at 4.14 2.15 0.028 glucosamine 6-phosphate N-acetyltransferase, putative (GO:0008080)
314.m00052_x_at 0.07 2.12 0.041 hypothetical protein
21.m00282_at 0.21 2.08 0.038 hypothetical protein
528.m00022_at 0.10 2.03 0.018 hypothetical protein
48.m00192_at 0.17 2.01 0.013 hypothetical protein
  1. a763.m00027_s_at also represents 190.m00085 (hypothetical protein), 239.m00065 (hypothetical protein), 278.m00067 (hypothetical protein), 330.m00074 (hypothetical protein), 43.m00171 (hypothetical protein) and 458.m00061 (hypothetical protein)
  2. b36.m00220_s_at also represents 36.m00212 (hypothetical protein)
  3. c86.m00156_s_at also represents 119.m00135 (glutamine cyclotransferase, putative)
  4. d93.m00158_s_at also represents 153.m00091 (DNA mismatch repair protein mutS, putative)
  5. e472.m00058_s_at also represents 361.m00052 (hypothetical protein)
  6. f289.m00067_s_at also represents 180.m00106 (short chain dehydrogenase family protein)
  7. g54.m00234_s_at also represents 169.m00132 (hypothetical protein) and 406.m00049 (hypothetical protein)
  8. h48.m00224_s_at also represents 309.m00047 (acetyltransferase, putative)
  9. The probe set, baseline expression value in untreated E. histolytica HM-1:IMSS trophozoites, fold change in 5-AzaC treated parasites, p-value, and gene annotation are shown. Genes in bold are those for which RT-PCR confirmation was performed. Genes in italics are genes whose expression is significantly higher in E. histolytica Rahman compared to E. histolytica HM-1:IMSS (p-value 0.0002). Genes with normalized expression values >0.20 can routinely be detected by RT-PCR. Genes marked by * are those for which bisulfite sequencing was performed. For annotations, GO IDs are given where available and are provided within parentheses.
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