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Table 3 A subset of genes down-regulated in 5-AzaC treated E. histolytica HM-1:IMSS parasites as compared to untreated controls.

From: Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression

Probe set Baseline expression Fold-change p-value Annotation
Potentially virulence associated
2.m00545_at* £ 4.12 0.27 0.033 cysteine proteinase, putative (GO:0006508)
52.m00148_at 0.96 0.46 0.018 lysozyme, putative
1.m00663_at 6.21 0.36 0.006 myosin calcium-binding light chain, putative (GO:0005509)
Protein kinases
35.m00259_at 1.76 0.48 0.013 protein kinase, putative (GO:0005524)
67.m00091_x_at£ 3.42 0.37 0.033 protein kinase, putative (GO:0005524)
99.m00190_s_at 0.39 0.46 0.040 protein kinase, putative (GO:0006468)
466.m00033_x_at 0.51 0.47 0.003 protein kinase, putative (GO:0005524)
264.m00068_at 2.29 0.44 0.029 protein kinase, putative (GO:0005524)
GTPases
106.m00140_at 2.39 0.48 0.025 Rap Ran GTPase activating protein, putative
2.m00606_x_at 0.20 0.36 0.005 Rab family GTPase (GO:0005524)
226.m00092_at* 0.34 0.44 0.008 Rab family GTPase
30.m00257_at 4.28 0.50 0.023 Rab family GTPase (GO:0005524)
34.m00273_x_at 3.22 0.43 0.040 Rab family GTPase (GO:0005524)
71.m00153_x_at£ 2.21 0.47 0.002 Rho family GTPase (GO:0005525)
8.m00366_s_ata 1.15 0.46 0.021 Rho GTPase activating protein, putative
171.m00089_at 4.88 0.35 0.044 GTPase activating protein, putative (GO:0003924)
Genes with decreased expression in E. histolytica (Rahman) compared to E. histolytica (HM-1:IMSS)
108.m00122_at 32.12 0.21 0.036 hypothetical protein
136.m00107_at 1.20 0.38 0.030 hypothetical protein
22.m00298_at 4.12 0.43 0.002 hypothetical protein
297.m00061_at 5.00 0.35 0.010 hypothetical protein
32.m00239_at 0.93 0.46 0.039 N-acetylglucosaminyl transferase, putative
37.m00215_at 1.90 0.46 0.035 hypothetical protein
442.m00023_x_at 0.82 0.44 0.006 hypothetical protein
460.m00024_s_atb 2.18 0.27 0.015 hypothetical protein
Genes overlap with day-1 & day-29 significantly up-regulated genes from in vivo mouse model (Gilchrist et al , 2006) £
15.m00302_at 4.05 0.41 0.030 hypothetical protein
2.m00545_at* 4.12 0.27 0.033 cysteine proteinase, putative (GO:0006508)
67.m00091_x_at 3.42 0.37 0.033 protein kinase, putative (GO:0005524)
71.m00153_x_at 2.21 0.47 0.002 Rho family GTPase (GO:0005525)
Miscellaneous
338.m00049_s_atc 4.30 0.42 0.020 LIM domain protein (GO:0008270)
10.m00349_x_at 4.30 0.44 0.024 paxillin, putative (GO:0008270)
35.m00253_at 1.74 0.26 0.036 iron-sulfur flavoprotein, putative (GO:0006118)
646.m00021_s_at 0.36 0.40 0.009 iron-sulfur flavoprotein, putative
90.m00179_at 1.31 0.46 0.044 amino acid transporter, putative (GO:0006865)
139.m00118_at 3.90 0.44 0.048 histone H3, putative (GO:0005634)
  1. a8.m00366_s_at also represents 58.m00170 (Rho GTPase activating protein, putative)
  2. b460.m00024_s_at also represents 353.m00048 (hypothetical protein) and 353.m00049 (hypothetical protein)
  3. c338.m00049_s_at also represents 321.m00056 (LIM domain protein)
  4. The probe set, baseline expression value in untreated E. histolytica HM-1:IMSS trophozoites, fold change in 5-AzaC treated parasites, p-value, and gene annotation are shown. Genes are clustered according to functional categories. Genes in bold are those for which RT-PCR confirmation was performed. Genes with normalized expression values >0.20 can routinely be detected by RT-PCR. Genes marked by * are those for which bisulfite sequencing was performed. Genes that were significantly upregulated in E. histolytica HM-1:IMSS trophozoites passed through mice are obtained from Gilchrist et al [27] and are indicated by £. For annotations, GO IDs are given where available and are provided within parentheses.