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Table 1 QPCR validation analysis of select neuronal mRNAs.

From: Transcriptional profiling of C57 and DBA strains of mice in the absence and presence of morphine

 

C57 Control

C57 Morphine

DBA Control

DBA Morphine

Arpc5*+!

1.00 0.13

1.42 ± 0.19

5.48 ± 0.38

13.61 ± 0.23

Comt*

1.00 ± 0.09

1.68 ± 0.39

0.71 ± 0.20

0.64 ± 0.05

Gabra2

1.00 ± 0.20

2.38 ± 0.34

3.99 ± 0.40

2.67 ± 0.62

Gnb1*

1.00 ± 0.10

1.46 ± 0.36

7.85 ± 0.22

12.13 ± 0.12

Nab2!

1.00 ± 0.10

1.28 ± 0.33

1.25 ± 0.36

0.41 ± 0.05

Pde9a*

1.00 ± 0.05

1.03 ± 0.06

2.04 ± 0.41

2.66 ± 0.31

Pitpnb*

1.00 ± 0.07

0.94 ± 0.05

0.34 ± 0.42

0.37 ± 0.15

Rcn+!

1.00 ± 0.03

6.18 ± 0.92

3.27 ± 0.81

1.47 ± 0.27

Sgne1*

1.00 ± 0.12

0.99 ± 0.07

3.88 ± 0.32

2.59 ± 0.19

  1. Nucleus accumbens (NAC) from vehicle or morphine (4 d) treated C57 or DBA mice were dissected and used for QPCR analysis. Eight neuronal mRNAs are profiled above, showing for each mRNAs the expression in C57 control, C57 with morphine, DBA control, and DBA with morphine, always normalized to the average expression in C57 control. Data represent means ± SEM of expression normalized to the average expression in C57 under control conditions. *, P < 0.05 for main effect of strain; +, P < 0.05 for main effect of treatment; !, P < 0.05 for interaction.
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BMC Genomics

ISSN: 1471-2164

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