SAMD9 protein is expressed and localized to the cytoplasm. A. Western analysis for the SAMD9-EGFP fusion protein. The SAMD9 fusion protein was detected as a predicted 200 kDa band in Cos-1 cells, using an antibody to GFP. B, C. Expression of SAMD9 putative protein in MRC-5 cells. N-terminal EGFP tagged SAMD9 was detected in the cytoplasm of MRC-5 cells when transiently transfected. B. Light field view. C. Fluorescent microscopy view for FITC. D, E, F. Expression of SAMD9 putative protein in SW480 cells. N-terminal EGFP tagged SAMD9 localized to the cytoplasm of SW480 cells. D. Fusion protein detected under fluorescent wave length for FITC. E. Cell nucleus detected under fluorescent wave length for DAPI. F. Merged view of image D and E.