Comparative genomics and experimental promoter analysis reveal functional liver-specific elements in mammalian hepatic lipase genes

Table 1 Pairwise sequence comparison of the coding and the 5'-flanking sequence of mammalian HL genes.

	upstream regulatory sequence
cDNA sequence	81		53	53	61	57	56	hedgehog^a
	79	76		86	64	55	59	mouse
	79	76	92		63	57	57	rat
	85	82	79	79		94	94	rhesus^a
	85	82	80	80	96		98	chimp
	85	82	79	79	96	99		human
	rabbit^b	hedgehog	mouse	rat	rhesus	chimp	human

Sequence identity (%) was determined by pairwise alignment using the DNAMAN software package (optimal alignment; gap open penalty 10.0, gap extension penalty 5.0). cDNA sequence was from the translation start ATG up till the stopcodon; the upstream regulatory region was from -1400 up till the translation start ATG.
^a: Because of a gap in upstream regulatory region of the public sequence of the rhesus monkey and hedgehog HL gene (Ensembl e ! 42: Dec 2006), alignments with the rhesus and hedgehog sequence were done with the sequence from -900 and -1000 up till the start ATG, respectively.
^b:the rabbit HL cDNA sequence was taken from [38].

ISSN: 1471-2164