Figure 3

Heteoplasmic features of HTR arrays in halibut mtDNA. (A) Schematic map of the analysed HTR and flanking regions. The HTR arrays were amplified using a forward primer (FP) and a reverse primer (RP). Here, primer sets L16293/H17347 and L16376/H17369 were used on Atlantic/Pacific halibuts and Greenland halibut, respectively. (B) Separation of amplified products in a 2.5% agarose gel. M, the size marker 1 Kb Plus DNA Ladder from Invitrogen (right). Number of repeat motifs is indicated (left). The four smallest amplified fragments from Hh-2 (marked a, b, c, and d) were eluted from the gel and further sequence analysed. (C) Summary of sequence analyses of fragment a-d from Hh-2. Note that fragment a contains three repeats of the 61-bp motif (I*, II, II), fragment b contains two repeat motifs (II, II), fragment c contains a single motif (II), and fragment d lacks a complete motif. Motif I* is not present in the plasmid cloned array of Hh-2 (Figure 2) and may represent site heteroplasmy at array motif position 33.