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Table 2 Measurement of genotype and/or ACTH effects on transcript accumulation by relative quantitative real-time PCR and comparison with microarray data

From: Gene array and real time PCR analysis of the adrenal sensitivity to adrenocorticotropic hormone in pig

Gene symbol Microarray       Real time PCR      
  p-value (ANOVA)   Fold Change    p-value (ANOVA)   Fold Change   
     genotype: MS/LW treatment: ACTH/control    genotype: MS/LW treatment: ACTH/control
  genotype treatment interaction control ACTH LW MS genotype treatment interaction control ACTH LW MS
Rnf2 2.81E-13 7.89E-01 1.96E-02 3.5 2.5 1.2 -1.2 0.82 0.64 0.81 1.1 -1.0 1.1 1.0
Anpep 5.65E-12 8.27E-03 NS 2.1 1.9 -1.1 -1.2 < 0.0001 0.040 0.056 3.9 3.0 -1.0 -1.4
Gadd45b 6.38E-04 1.41E-11 NS -1.3 -1.3 2.4 2.4 0.028 < 0.0001 0.33 -1.5 -1.5 2.6 2.6
Eif1b 9.08E-06 7.89E-10 NS 1.5 1.9 2.5 3.3 0.009 < 0.0001 0.040 1.4 1.9 2.9 4.1
Crem 4.34E-02 2.70E-10 NS 1.7 1.1 4.4 3.0 0.18 < 0.0001 0.43 1.6 1.3 6.4 5.4
A2m 2.45E-10 3.68E-03 NS -1.8 -1.7 -1.2 -1.1 < 0.0001 0.11 0.54 -2.1 -2.1 -1.3 -1.2
Ldlr* 5.19E-03 4.15E-07 NS 1.6 1.3 2.3 1.8 ND ND ND ND ND ND ND
Ckb 1.03E-06 3.00E-02 NS 2.1 1.9 1.3 1.2 0.004 0.43 0.88 2.3 2.1 1.3 1.2
Dab2 2.92E-02 5.63E-05 NS -1.1 -1.3 -1.4 -1.7 0.34 0.052 0.63 -1.3 -1.2 -1.8 -1.6
Ldlr* 1.50E-02 1.14E-03 1.06E-02 1.5 1.0 1.6 1.1 0.069 < 0.0001 0.80 1.4 1.2 2.2 2.0
Fxc1 3.06E-04 2.35E-02 NS 1.2 1.3 1.1 1.1 0.28 0.16 0.48 -1.1 -1.4 1.6 1.2
Star 4.85E-04 NS NS 1.6 1.2 1.4 1.1 0.053 0.26 0.406 1.4 1.1 1.3 1.0
Mc2r ND ND ND ND ND ND ND 0.95 0.24 0.77 -1.1 1.0 1.2 1.4
Scarb1 NS NS NS NS NS NS NS 0.89 0.25 0.75 1.1 -1.0 1.2 1.1
Bzrp ND ND ND ND ND ND ND 0.017 0.64 0.089 -2.3 -1.2 -1.4 1.4
Sqle NS NS NS NS NS NS NS 0.16 0.39 0.32 -1.1 -1.5 1.3 -1.0
Cyp11a1 NS NS NS NS NS NS NS 0.85 0.36 0.37 1.2 -1.1 1.4 1.0
Hsd3b1 NS NS NS NS NS NS NS 0.51 0.17 0.94 -1.1 -1.1 -1.3 -1.3
Cyp21 NS NS NS NS NS NS NS 0.022 0.90 0.33 -1.2 -1.5 1.1 -1.1
Cyp11b NS NS NS NS NS NS NS 0.13 0.27 0.94 1.3 1.3 1.2 1.2
Per2 ND ND ND ND ND ND ND 0.46 0.040 0.066 -1.2 1.8 -2.3 -1.0
Cry1 NS NS NS NS NS NS NS 0.96 0.48 0.62 1.1 -1.1 1.2 1.0
Bmal1 ND ND ND ND ND ND ND 0.002 0.21 0.70 -2.5 -2.9 -1.4 -1.6
  1. The changes in expression levels for 11 genes shown by microarray analysis to be significantly regulated by genotype and/or ACTH were quantified by real-time PCR. For comparison, relative expression values for each gene were determined in each animal (fold change, n = 6 pigs per experimental point). Changes in expression levels were also analyzed for 11 additional genes of great interest for our study whereas they were either shown by microarray analysis to be not significant (NS, p > 0.05) or not determined (ND) since they were not included on the microarray or due to the data normalization process. * LDLR gene was represented by 2 cDNA clones on the microarray and changes in its expression were verified by real-time PCR using only the cDNA sequence for which exons/introns structure could be deduced.