Figure 3

The ERF gene family of cowpea transcription factors. GSRs encoding the conserved DNA binding domain of ERFs were identified, the 111 cowpea ERF genes were arbitrarily assigned names, and the conserved domains were aligned using ClustalW. An unrooted phylogenetic tree was produced using the PHYLIP program based on the neighbor-joining method and presented using PhyloDraw. The cowpea ERF family is separated into two major clades. A line divides the CBF/DREB subfamily from the ERF subfamily. Subgroups, indicated by roman numerals, were identified as described in [58]. For additional information see Additional file 5 and Additional file 6.