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Figure 6 | BMC Genomics

Figure 6

From: Search for the genes involved in oocyte maturation and early embryo development in the hen

Figure 6

Ribosomic and messenger RNA profiles during oocyte maturation and early embryo development. Total RNA was extracted from the GDR of an oocyte or from the embryo. RNA quality was assessed using nanochips (Agilent technologies) as described in the Materials and Methods. This analysis represents rRNA 18S and 28S subunit profiles in the samples (A). Ribosomic RNA 18S and 28S subunit profiles were then confirmed by real time PCR analysis (B and C, respectively) using the TaqMan kit (Eurogentec) as described in the Materials and Methods. Labelled 32P reverse transcription was performed to investigate the quality of mRNA extracted from F1 GDR, Ov GDR and from the ovary. The radioactive signal for samples and ethidium bromide staining for the ladder are shown (D).

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