Figure 2

Microarray experimental design. A. Loop design used for microarray hybridization study. Each RNA sample was divided into 2 parts: one was labeled with Cy3 and the other with Alexa 647. Two cDNAs with different dyes were paired and hybridized to an array. B. For each paired sample, dye flip was used to overcome the difference between two fluorescent dyes because their intrinsic properties. Three cell preparations were used.