Table 6 Primer sequences used for RT-PCR
Representative clone | Â | Primer sequence | Product size (bp) |
|---|---|---|---|
Highly expressed in BPH | |||
MB3851 | forward | GGCGTGTTGGTGTTGCTTCA | 515 |
| Â | reverse | GCTTAGCAAATCCTGGGACA | Â |
Female gonad-specifically expressed | |||
AB0551 | forward | AATCATCGCTGCTTTCCTCGCC | 437 |
| Â | reverse | GGGAGCAAGGAGAGGCTATTAC | Â |
OB0570 | forward | GGGACACAGACTTAACTTGGCG | 505 |
| Â | reverse | AGCAACTCTTGCTCCATAGT | Â |
OB0998 | forward | TCCCAAAGTCGATGGCCGAATC | 529 |
| Â | reverse | TGGTACTGACTGATCGTTCC | Â |
OB1013 | forward | GACGGAGCAACCGTACATAT | 486 |
| Â | reverse | CGGTGAAGTAGATGGTCTCA | Â |
OB1061 | forward | CATCAGCCCTGTGACGATAGAG | 602 |
| Â | reverse | TCTGCACTCGCTGCTGTTCCAT | Â |
OC0606 | forward | CTGTGATCTCAAGCTCAGCCTG | 498 |
| Â | reverse | CACCAGCATGACCTTGGAGTAC | Â |
OC0638 | forward | GTCTGTGCCCTTTGCGCCAT | 504 |
| Â | reverse | GCATGGCTGATGGCAGACTG | Â |
OC0681 | forward | GTAAAATGGCCGCCAAGTCACT | 435 |
| Â | reverse | GAATGGAGAGGTGTGTTGCA | Â |
OC1014 | forward | CGTTGTTGCTGCCTTGGCACTA | 480 |
| Â | reverse | CCTGGATTTGCCTAAGCTGCAA | Â |
OC1083 | forward | CATCCTGTGGATGCGGATACTC | 512 |
| Â | reverse | GACTAGTAAAGCACAGATCG | Â |
OC2756 | forward | AGTGATCACAAGCTCTGCCTG | 382 |
| Â | reverse | TATGCCACACAGCCAACACCAG | Â |
Male gonad-specifically expressed | |||
AC0657 | forward | CGAGCTCTGTGTTGCTGAAG | 575 |
| Â | reverse | CAGCAGGAGTTAAAACATGGG | Â |
AC0743 | forward | CACCAACTCAACACTCCAC | 525 |
| Â | reverse | TTACTTCGGCCACACGGCTC | Â |
AC0825 | forward | GCGACTGGTGCAAAGTAGCA | 610 |
| Â | reverse | TCTTGACTCAGCTTCCCATC | Â |
AD1216 | forward | CTTCATTGTGGCTGTTGCAAG | 444 |
| Â | reverse | TCAGGGTATAGTGACCTTCC | Â |
NB2328 | forward | CCTCAGTACACAGTGAAACTC | 480 |
| Â | reverse | GCTCTTCACAGGGATCCACA | Â |
NB3697 | forward | GGCAGAATCAGTCGAAGATC | 435 |
| Â | reverse | GCGCCACATTTTGGAATGGG | Â |
NB5623 | forward | AGAACCAAACCCAAACAGGC | 416 |
| Â | reverse | GTTACACGGATACAAACCCTG | Â |
TA0293 | forward | ACCATGCAGCAGAGGGAATC | 542 |
| Â | reverse | GGATGGGTGACCATGGAGTC | Â |
TA0428 | forward | CCATCTCCTGAAACTGTAGG | 464 |
| Â | reverse | AGGCAGTTCAAGTGGCTCTC | Â |
TA0528 | forward | CCAAAACTCCTACGGTACCG | 502 |
| Â | reverse | TTTTTCGCAGGGGTCGGTGC | Â |
TA0721 | forward | CACTCATCTCCAGTGATACC | 452 |
| Â | reverse | CTGGTGCTAATGTTGGAGCG | Â |
TA1050 | forward | TCATTTTCCCGATGGCAAGC | 527 |
| Â | reverse | TTTCGACGGCACGCTTCTCC | Â |
TA1095 | forward | CCAGAGATCCTGCAGAGACA | 545 |
| Â | reverse | GCCTTAGTGTAACACTATTCCC | Â |
Control PCR primer for template validation | |||
NL_EF2 | forward | GAAGTTCAGTGTGTCGCCTG | 420 |
| Â | reverse | TCTCCCAGATATCTGGCTCT | Â |