Figure 6
From: Bioinformatics prediction of overlapping frameshifted translation products in mammalian transcripts
Thrombopoeitin matreshka. A) mRNA sequence of thrombopoietin (THPO), with matreshka underlined. 5'UTR sequence is shown in lowercase, Kozak motifs in bold. The bold white guanine base (grey background) can be replaced by adenine to truncate the matreshka through a SNP (NCBI ID rs1126665), and replaces a glycine with a glutamate within the Erythropoietin-Thrombopoietin motif (Pfam ID PF00758.7). Alternating italics/non-italics represent alternate THPO coding exons, and spaces separate codons for the parent RefSeq. B) matreshka protein sequence. C) Illustration of the translation of the THPO matreshka in the nirs splice variant. THPO splice variant 1 is shown above the nirs splice variant. Hatching indicates the location of the matreshka. Nirs splicing utilises an alternative acceptor which results in the last exon being translated in the same frame as the matreshka over 564 nucleotides (indicated by grey shading).