Figure 17

Multiple sequence alignment of VWA domain – Hint-like domain proteins, part 1. Proteins containing a VWA merged to a Hint-like domain were discovered in Tetrahymena, several fungal species, as well as several other eukaryote branches, including choanoflagellates. The VWA domain and the Hint-like domain (Vint) with motifs A and B of the Hint domain are marked in the alignment. A new domain between the VWA and Vint domain is marked with Vwaint. Four proteins also have an Ubox upstream of the VWA domain. An alignment of selected Vint domains to Hh Hog domains is presented in additional file 15. A. thaliana At5g60710 is not a Vint protein, but one of the best matching VWA domain containing proteins. While it lacks the Vint domain, it does have some weak similarity to the Vwaint domain, and upstream of the VWA domain is a Ring finger, which shares similarity with the Ubox motif. It would be worthwhile to investigate this similarity with a detailed evolutionary analysis in the future.