Figure 3

RT-PCR analyses for representative GACA- (A) and GATA- (B) tagged transcripts using internal primers and cDNA from different somatic tissues, gonads and spermatozoa as templates. The transcript IDs are given on the left and names of the tissues on the top. Quality and quantity of the cDNA samples was normalized (C) and genomic contamination in the RNA checked by PCR with β-actin derived primers. Tissue specificities of the transcripts were ascertained on the basis of presence or absence of amplicons using the respective cDNA templates which were further confirmed by real time PCR and Southern blotting.