Table 2 Parameter settings relaxed for round two in the primer design process.
Product length, round 2 | 80 – 160 nucleotides |
Primer melting temperature, round 2 | 50.0 – 60.0 ° |
Max. base repeat, round 2 | 3 (e.g., GGG in a primer is allowed) |
Product length, round 2 | 80 – 160 nucleotides |
Primer melting temperature, round 2 | 50.0 – 60.0 ° |
Max. base repeat, round 2 | 3 (e.g., GGG in a primer is allowed) |