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Table 1 Selected list of modulated grape transcripts.

From: cDNA-AFLP analysis of plant and pathogen genes expressed in grapevine infected with Plasmopara viticola

TDF Primer comb. Accession Lenght (bp) I/R Annotation Blast score Blastn/Blastx*
Metabolism
192 TC – CA TC52910 301 + Sucrose synthase (Q9SLS2) 3.12E-63
1627 TT – CT TC57852 168 + UDP-glucosyltransferase HRA25 (Q9FUJ6) 2.07E-30
245 TA – AC TC53221 169 + Transketolase, chloroplast precursor (O20250) 1.92E-29
18 TT – AA TC67193 127 + Raffinose synthase, partial (Q8VWN6) 9.26E-21
1279 CT – TC TC52362 175 + Fatty acid multifunctional protein (Q9ZPI5) 1.12E-16
134 TC – TT TC67104 390 + 12-oxophytodienoate-10,11-reductase 1 (Q8LAH7) 6.19E-24
993 CA – CA TC60564 50 + Gibberellin 2-oxidase (Q6TN17) 3.63E-105
215 TT – TA TC55722 216 + Fatty acid hydroperoxide lyase (Q9AUD8) 5.62E-72
152 TC – TC Q6X5R6 342 + (Lox2) Lipoxygenase 4.00E-39 *
1036 CC – AT TC58112 349 + Long-chain acyl-CoA oxidase (O64894) 5.62E-72
1225 CT – AC TC53311 359 + 3-ketoacyl-CoA thiolase (Q6TXD0) 2.10E-09
893 CA – AC TC67959 104 + 3-beta-hydroxy-delta5-steroid dehydrogenase 9.77E-16
347 TA – CG TC54708 181 + 40S ribosomal protein S16 (Q9M5L1) 2.91E-18
1382 CG – TA TC58494 279 + Ribosomal L10 protein (Q874B2) 2.62E-56
1345 CG – AA TC51894 289 + Ubiquitin conjugating enzyme E2 (Q42897) 6.56E-55
594 TG – GA TC60588 186 + Proteasome subunit beta type 5-B precursor (Q9LIP2) 5.35E-37
1493 CT – GG TC56558 399 + Gamma-glutamylcysteine synthetase (Q6F4I8) 4.64E-35
1293 CT – TG TC51806 244 + Cysteine synthase (Q43317) 3.08E-25
79 TC – AC TC68684 457 + Cellulose synthase-like protein D4 (Q8GUZ9) 2.09E-96
1594 TT – TC TC65238 179 + Tubulin alpha chain, partial (P33629) 4.78E-34
232 TA – AT TC57434 161 - Nucleotide sugar epimerase-like protein 8.83E-12
1630 TT – CT Q9ZTP5 105 - Pentose-5-phosphate 3-epimerase 5.85E-10 *
1610 TT – CA TC54570 167 - Sedoheptulose-bisphosphatase (Q940F8) 1.37E-27
1668 TT – GT TC54602 412 - Fructose-bisphosphate aldolase (Q6RUF6) 1.47E-50
100 TC – AG TC54851 224 - Alpha-mannosidase (Q2R3E0) 9.53E-43
1567 CG – GT TC57827 211 - Galactose dehydrogenase, (Q84LI1) 4.13E-33
255 TA – AG TC52686 210 - Carbonic anhydrase (Q5NE20) 2.18E-39
1472 CT – GC TC60916 581 - Glyceraldehyde-3-phosphate dehydr. B subunit 1.61E-126
1205 CT – AT TC62475 182 - Lipase class 3-like (Q6K2K7) 6.71E-33
1689 TT – GG TC53435 141 - B-keto acyl reductase (O24479) 4.93E-24
670 CA – GA TC62496 145 - 3-hydroxy-3-methylglutaryl coenzyme A (Q8W2E3) 6.52E-12
151 TC – TC Q8H539 358 - Steroid 5alpha-reductase-like protein 1.00E-32*
1239 CT – AG TC69679 98 - 60S ribosomal protein L19 (Q6RYC4) 1.65E-13
138 TC – TT TC59193 99 - Protein translation factor SUI1 homolog 2 (Q94JV4) 1.49E-12
308 TA – CA TC51783 621 - Elongation factor 1-beta 1 (Q84WM9) 1.99E-64
823 TC – GG TC54220 542 - Phytocalpain (Q6SSJ2) 3.95E-39
1174 CC – CC TC63107 115 - Chaperone protein dnaJ-like (Q6H3Y3) 9.58E-18
1530 CC – GC CB348741 62 - F-box protein family AtFBL5 1.37E-12
1476 CT – GC CA816379 345 - Protein At3g07360 (U-box domain-containing prot.) 2.37E-69
1389 CG – TT TC65574 203 - Glycin-rich protein (Q43688) 3.13E-35
1546 CG – GA TC68519 541 - Expansin, complete (Q84US7) 8.40E-112
722 TC – CT TC69230 350 - Cyclin D1, partial (Q8GVE0) 1.73E-69
664 CA – GA TC53870 228 - Integral membrane protein-like (Q5VRH) 7.89E-07
Photosyntesis and Energy
1330 CT – CC TC61438 646 + Cytochrome P450 monooxygenase (Q2MJ14) 1.38E-67
123 TC – TA TC68921 64 + C-type cytochrome biogenesis protein (Q7XY14) 1.08E-06
227 TA – AT TC62259 353 - ATP synthase B' chain, chloroplast precursor 1.03E-66
749 TC – CG TC63430 230 - NADPH-cytochrome P450 oxydoreductase 1.20E-29
520 TG – TG TC65998 102 - Plastocyanin, chloroplast precursor (P17340) 6.20E-06
1247 CT – TA TC66316 365 - Chloroplast photosystem II 10 kDa protein (Q2PXN6) 5.90E-58
1156 CC – CT TC68056 154 - NADH-plastoq. oxidoreductase subunit 7 (Q2L953) 2.39E-26
1049 CC – AC TC53584 561 - Magnesium chelatase subunit, partial 1.28E-121
31 TT – AT TC55659 163 - Chlorophyll A/B binding protein precursor (Q32291) 1.79E-32
427 TG – TA TC57132 209 - Chlorophyll A/B binding protein, precursor (P13869) 3.62E-42
4 TT – AA TC55138 381 - Photosystem I assembly protein ycf3, partial 2.10E-78
1115 CC – TC TC61693 411 - Photosystem I reaction center subunit II (P12372) 2.51E-84
1322 CT – CT TC66994 275 - Photosystem II reaction center (Q8W536) 2.26E-52
161 TC – TC TC58978 146 - Photosystem II M protein (Q6QXV8) 3.80E-25
1393 CG – TT TC58567 103 - Apocytochrome f precursor (Q68RZ3) 4.92E-10
Transport
1614 TT – CA Q3L7K6 185 + Hexose transporter 6.26E-08 *
680 CA – GT TC52346 231 - Transporter-like protein (Q9LSH7) 3.88E-47
249 TA – AC TC66367 107 - 14-3-3 protein (Q93XW1) 1.13E-16
1534 CC – GG TC57372 435 - Nitrate transporter NRT1-2 (Q9FRU5) 8.33E-94
1438 CG – CC TC65826 270 - ABC transporter-like protein (Q9FT51) 7.38E-50
133 TC – TT TC62785 219 - Calcium-transporting ATPase 8, plasma membr. 4.00E-23
662 CA – GA TC69251 302 - ATPase alpha subunit, complete (Q3C1H4) 7.47E-59
1679 TT – GC BQ798655 127 - Syntaxin {Glycine max} 5.39E-23
792 TC – GT TC51721 180 - ADP-ribosylation factor 1-like protein (Q70XK1) 2.22E-36
62 TT – AG TC69827 195 - Aquaporin PIP2 (Q2HZF5) 1.45E-20
Signal transduction
434 TG – TA TC59460 147 + Protein phosphatase 2C (Q8RVG0) 6.61E-23
240 TA – AC Q52QR5 343 + NAC domain protein NAC1 1.70E-71*
1385 CG – TA TC55407 95 + NAC family protein (Q2Z1Y1) 2.32E-13
687 CA – GT TC70344 687 + WRKY DNA-binding protein 32 (P59583) 1.24E-05
235 TA – AT TC52484 102 - SOS2-like protein kinase (Q8LK24) 1.58E-12
1298 CT – TG TC60214 164 - Calcium-transporting ATPase/calmodulin binding 2.74E-28
288 TA – TC TC52400 212 - Calmodulin cam-207 (Q6DN29) 1.23E-39
1538 CC – GG TC66781 267 - CNGC2 (Q5D6H2) 1.22E-47
633 TG – GC TC69501 633 - Calcium sensor calcineurin B-like protein (Q4W3B4) 1.02E-34
11 TT – AA TC61968 289 - Protein At1g01140 (CBL-interacting protein kinase) 7.34E-54
778 TC – GA TC53469 55 - MAPKK (Q66MH6) 8.34E-07
1573 CG – GC TC59576 700 - Receptor-like kinase with LRR repeats (Q70AH8) 1.44E-32
1231 CT – AG TC52043 339 - MADS-box transcription factor FBP29 (Q9ATE2) 2.44E-72
Secondary metabolism
1539 CC – GG TC52853 419 + Cinnamyl alcohol dehydrogenase (Q2Z1Z0) 2.21E-08
909 CA – AG TC53668 105 + Stilbene synthase [Vitis vinifera] 5.34E-17
1249 CT – TA TC54354 273 - Secretory laccase (Q6TDS6) 1.11E-23
421 TG – AG TC51729 113 - Caffeoyl-CoA O-methyltransferase (Q43237) 5.87E-17
362 TG – AA TC53331 208 - Flavanone 3-hydroxylase-like protein, (Q9FLV0) 2.88E-34
432 TG – TA TC65435 179 - Limonoid UDP-glucosyltransferase (Q9MB73) 1.29E-32
Defence response
1176 CC – CG TC63410 557 + Beta-1,3-glucanase (Q9M563) 5.95E-120
68 TT – AG TC56756 108 + Pathogenesis-related protein 10.3 (Q20BD2) 6.31E-14
724 TC – CT TC56512 253 - Pollen allergen-like protein, partial {A. thaliana} 1.09E-49
1296 CT – TG TC62916 157 - EXECUTER1 protein (Q93YW0) 1.72E-27
692 CA – GC TC57989 251 - Avr9/Cf-9 rapidly elicited protein 276 (Q84QD7) 3.72E-23
229 TA – AT TC70153 282 - Flax-inducible sequence 1 (Q40255) 6.91E-56
1173 CC – CC TC51855 262 - Pto-like serine/threonine kinase (Q6W0C7) 1.69E-16
1545 CG – GA TC58939 671 - Ethylene-resp. element-binding factor (Q2QDF5) 3.13E-125
584 TA – GC TC62111 83 - Lipid transfer protein (Q93YX9) 4.49E-10
889 CA – AC TC63540 305 - DIR1 (At5g48485) {Arabidopsis thaliana} 2.98E-58
214 TT – TA TC61755 233 - Enzymatic resistance protein, complete (Q3S4G9) 1.61E-44
Response to stimulus
246 TA – AC TC63756 153 + Metallothionein-like protein (Q3HR41) 3.69E-27
945 CA – TT TC53817 79 + Thioredoxin H, (Q4U0W0) 1.68E-08
1275 CT – TC TC53088 383 + Glutathione S-transferase GST 24 (Q9FQD4) 7.96E-81
748 TC – CG TC54876 240 + Ferritin-3, chloroplast precursor (Q948P6) 2.59E-47
1631 TT – CT Q6V7W6 84 + Class III peroxidase GvPx2b 2.76E-04
1245 CT – TA TC62299 380 - Auxin-induced SAUR-like protein (Q8S351) 1.89E-79
1562 CG – GT TC53184 397 - Chloroplast small heat shock protein (Q6WHC0) 1.14E-80
781 TC – GA TC53791 232 - Catalase (Q7XTK9) 4.25E-45
532 TG – CT TC56223 206 - Peroxiredoxin Q (Q6QPJ6) 4.38E-37
  1. I/R: induced or repressed in cDNA-AFLP experiments.