Impaired barrier function by dietary fructo-oligosaccharides (FOS) in rats is accompanied by increased colonic mitochondrial gene expression

Table 3 Q-PCR confirmation of microarray data.

			Micro-array	Q-PCR
Gene name	Gene symbol	Sequence ID	Ratio*	Ratio*	SEM ±	p-value†
Mitochondria
Complex I
NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9	Nduf9b	XM_216929	1.47	1.29	0.05	0.001
Complex II
Succinate dehydrogenase complex, subunit B, iron sulfur (Ip)	Sdhb	XM_216558	1.43	1.32	0.05	<0.001
Complex III
Ubiquinol-cytochrome c reductase binding protein	Uqcrb	XM_001074024	1.53	1.28	0.05	0.005
Complex IV
Cytochrome c oxidase subunit VIIb	Cox7b	NM_182819	1.61	1.36	0.06	0.001
Complex V
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G	ATP5g	XM_001075306	1.52	1.15	0.05	0.11
Metabolism and TCA cycle
Aldo-keto reductase family 1, member B8	Akr1b8	NM_173136	2.13	2.15	0.22	0.006
Malic enzyme 1	Me1		1.65	1.76	0.11	<0.001
Mitochondrial ribosomes
Mitochondrial ribosomal protein S16	Mrps16	XM_001064095	1.43	1.20	0.05	0.03
Mitochondrial protein transport
Translocase of inner mitochondrial membrane 8 homolog b	Timm8b	NM_022541	1.45	1.19	0.06	0.09
Protein turnover
Proteasome subunit alpha type 3-like	Psma31	BN000326	1.39	1.24	0.04	0.002
Mucosal barrier
Phospholipase A2, group IIA (platelets, synovial fluid)	Pla2g2a	NM_031598	3.73	4.70	0.87	0.03
Trefoil factor 3	Tff3	NM_013042	1.7	1.21	0.05	0.04
Top 10 gene
Retinol binding protein 7	Rbp7	XM_575960	2.06	3.72	0.36	<0.001
Peptide hormone
Proglucagon	Gcg	NM_012707	2.56	2.91	0.23	<0.001

* Ratio FOS diet/Control diet.
± SEM of Q-PCR data is given (n = 12 per group)
† p-value of Q-PCR data is given, the p-value of microarray data were all <0.001.

ISSN: 1471-2164