A) Immunofluorescence analysis of actin and β-tubulin staining in E15 primary cortical neurons of wt, Cln1-/- and Cln5-/- m ice. Actin is visualized in green and β-tubulin in red. The overlay shows the colocalization of the two proteins in yellow. B) Quantitative threshold imaging of actin and β-tubulin staining in the Cln5-/- mouse brain. Significant p-values, with p < 0.05 are indicated with *. Ctx, cortex and cc, corpus callosum.