Figure 4

Average third position GC (GC3) content for expression categories. Sex-biased expression was determined with absolute log₂ fold-change > 1 cutoff (p _adj < 0.05). 95% confidence intervals (based on bootstrapping, 1000 repetitions) are shown. Sex-biased categories that differed significantly from unbiased genes in the same tissue are indicated (*, p < 0.05, see materials and methods for statistical metrics). Pink bars represent female GC3 values, blue bars represent male values, and yellow bars represent unbiased values. The Y-axis represents the proportion of coding sequence composed of G or C.