Expression levels of pluripotency markers upon siRNA knockdown of the novel TUs. Nanog and Klf4 expression was reduced to 70% upon knockdown of TU7 but no down regulation of the other pluripotency markers was observed. There was no down regulation of Oct4, Sox2, Nanog and Klf4 upon knockdown of the other novel TUs. Expression level was measured relative to Non-Targeting siRNA control (100%). Error bars indicate S.E.M. from two biological replicates.