ClustalW alignment of ORF sequences of the eleven 65 kDa mGBP members. The alignment-layout was edited with the JalView software. Basepairs identical in all eleven mGBPs are marked in dark blue, identical in ≥75% in medium blue and identical in ≥50% in light blue. Red boxes highlight the conserved GTP binding motifs: P-loop (G1), Switch I (G2), Switch II (G3) and G4. This figure shows the upper quartile, for the full image please see additional file 2.