Conservation of IgR 1698 in pseudomonads. a. Alignment of the P. aeruginosa PAO1 IgR 1698 with homologous sequences present in P. fluorescens Pf0-1, P. putida KT2440, P. fluorescens Pf-5, P. syringae pv. tomato DC3000 and P. fluorescens SBW25. The IgR 1698 sequence of P. fluorescens CHA0, which was determined experimentally in this study, is added to the alignment. The alignment was made using ClustalW . Conserved nucleotides are marked with an asterisk. +1, putative transcription start; URS, putative upstream regulating sequence; -35 and -10, postulated sigma-38 (RpoS) promoter elements. The sequence replaced by a BamHI site in the rgsA
-lacZ fusion construct pME7234 is underlined. b. Organization of the 2.4-kb rgsA gene region in strain CHA0. The sequence of the downstream ORF PFL_1803 is shown in boldface with its translated sequence below. The 1698Fus2 primer employed to construct pME7234 is shown as an arrow, with the cloning BamHI site detailed. Part of the sequence of the rsgA
overexpressing construct pME7236 is shown in italics; the tac promoter (Ptac) is indicated by grey boxes and its transcription start by +1.