Figure 1

Gene expression profile analysis of Parp-1^+/+ and Parp-1^-/- activated T cells. (A) Venn diagram showing the numbers of PARP-1-dependent genes in T cells upon stimulation with anti-CD3 mAb alone or in combination with anti-CD28 mAb. (B) Expression maps including the genes dependent on PARP-1 in response to both anti-CD3 and anti-CD3 + anti-CD28 stimulation. Red indicates higher expression in Parp-1^+/+ T cells compared to Parp-1^-/- T cells (positive regulation by PARP-1) while green indicates higher expression in Parp-1^-/- T cells compared to Parp-1^+/+ T cells (negative regulation by PARP-1). Numbers on the left column indicate the fold-change expression in Parp-1^+/+ T cells compared to Parp-1^-/- T cells. (C) Real-time PCR analysis of representative genes. Samples were normalized according to Gapdh expression levels. The y-axis represents fold-change of activated versus resting cells for both Parp-1^+/+ (white bars) and Parp-1^-/- (black bars) T cells. Results represent the mean value ± SD of two independent experiments. The ratio of gene expression in Parp-1^+/+ over Parp-1^-/- cells (positive number) or the ratio of gene expression in Parp-1^-/- over Parp-1^+/+ cells (negative number) is indicated above each pair of bars.