Gene expression profile analysis of Parp-1+/+ and Parp-1-/- activated T cells. (A) Venn diagram showing the numbers of PARP-1-dependent genes in T cells upon stimulation with anti-CD3 mAb alone or in combination with anti-CD28 mAb. (B) Expression maps including the genes dependent on PARP-1 in response to both anti-CD3 and anti-CD3 + anti-CD28 stimulation. Red indicates higher expression in Parp-1+/+ T cells compared to Parp-1-/- T cells (positive regulation by PARP-1) while green indicates higher expression in Parp-1-/- T cells compared to Parp-1+/+ T cells (negative regulation by PARP-1). Numbers on the left column indicate the fold-change expression in Parp-1+/+ T cells compared to Parp-1-/- T cells. (C) Real-time PCR analysis of representative genes. Samples were normalized according to Gapdh expression levels. The y-axis represents fold-change of activated versus resting cells for both Parp-1+/+ (white bars) and Parp-1-/- (black bars) T cells. Results represent the mean value ± SD of two independent experiments. The ratio of gene expression in Parp-1+/+ over Parp-1-/- cells (positive number) or the ratio of gene expression in Parp-1-/- over Parp-1+/+ cells (negative number) is indicated above each pair of bars.