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Table 3 Real-time qPCR primers used in the study

From: Gene expression analyses in Atlantic salmon challenged with infectious salmon anemia virus reveal differences between individuals with early, intermediate and late mortality

Genes

Primers

GenBank

ISAV segment 6 a

5-AGGCCAAAAACGGAAATGGA-3

AF220607

 

5-CCGTCAGTGCAGTCATTGGTT-3

 

18S rRNA b

5-GCCCTATCAACTTTCGATGGTAC-3

AJ427629

 

5-TTTGGATGTGGTAGCCGTTTCTC-3

 

Galectin-3 binding prot c

5-CCAGACCAACAGTGTTCACTTCAGC-3

BX079375

 

5-ACGTGAAAGACATACCTGCCCTCAC-3

 

Galectin-like 1 c

5-CAGCAACCCTTCTTCAATCCGAGA-3

CA344100

 

5-TCTCCCTGTCACAGTGATGGTCTTC-3

 

Tripartite motif/ring finger protein c

5-TGTTCTGTTGCTCCGTCTGTCTGGA-3

CA376536

 

5-TTCAGCCAGCACGGTGTTTCTCTTC-3

 

Tyr-protein kinase FRK c

5-TAGACATGGCACCATGGACCCTC-3

CA349577

 

5-GGGTTCTTCAGTGCAGACAGCCA-3

 

STAT1 c

5-GAACATGGAGGAGTCCAATGGAAGC-3

CA343225

 

5-GGACCCTCATTTGATCTGTTGCCT-3

 

CCAAT/enhancer binding protein beta c

5-TACGTCCTGGGCTATCCTGAACTGC-3

CA348284

 

5-CCAGACGAACCGTTGTTGTCCA-3

 

Beta-2-microglobulin c

5-TCGTTGTACTTGTGCTCATTTACAGC-3

AF180478

 

5-CAGGGTATTCTTATCTCCAAAGTTGC-3

 

IgM heavy chain (CH) d

5-ATACGGTGACCCTGACTTGCTACGT-3

S48652

 

5-TTCTCTCCACCGGCTCATCATCA-3

 

IgZ CH-like d

5-AGCACCAGGGACAAACCACCAT-3

CA372094

 

5-TTCACACTCGGTGGGTTCAGAGTC-3

 

IgD CH d

5-TCTTCAGGAGCTGAGGACAGATGGA-3

AF278717

 

5-AAACCCATCCACCTTCCAGCTGA-3

 

CD4 d

5-TGCATTGTTCCTCTCTTCCACAGC-3

AY973028

 

5-CCGTCCCAAGGTACCATAGTACCAA-3

 

TGF-beta d

5-AATCGGAGAGTTGCTGTGTGCGA-3

EU082211+

 

5-GGGTTGTGGTGCTTATACAGAGCCA-3

AJ007836

CD8-alpha d

5-CGTCTACAGCTGTGCATCAATCAA-3

AY693391

 

5-GGCTGTGGTCATTGGTGTAGTC-3

 

Interferon-gamma d

5-TTCAGGAGACCCAGAAACACTAC-3

AJ841811

 

5-TAATGAACTCGGACAGAGCCTTC-3

 

GRB2-related adaptor protein 2 e

5-TGACTTTACTGCCACTGCTGAGGAC-3

CA353121

 

5-CAGTCATCATTGGTGCCCAAGATC-3

 

Annexin A1-1 e

5-CTCCAGGAAATTGAACACCGCGA-3

CA364941

 

5-AAGGCTGCGATGAAGGACATGGT-3

 

5-lipoxygenase activating protein e

5-TCTGAGTCATGCTGTCCGTAGTGGT-3

CA369467

 

5-CCTCCCTCTCTACCTTCGTTGCAAA-3

 

Regulator of G-protein signaling 1-1 e

5-GACTCCTAACCTCCAATGCTTCGAC-3

CA383094

 

5-CGAATCTCTCTCCATCAGCCCATA-3

 

Cytochrome P450 2K4-1 e

5-TTCACCCTCCACCCTTCACCTC-3

EV384586

 

5-ATCTCAGACCCGGCTCACAGCA-3

 

Galectin-9 e

5-TCGCTGATTGTGAATGGTGCTCAC-3

CX035552

 

5-CAGGGTTGGAGAAGGCAATGGATT-3

 

Heat shock protein HSP 90-beta-2e

5-GAACCTCTGCAAGCTCATGAAGGA-3

BX074486

 

5-ACCAGCCTGTTTGACACAGTCACCT-3

 

Cathepsin S e

5-CGAAGGGAGGTCTGGGAGAGGAAT-3

CA355014

 

5-GCCCAGGTCATAGGTGTGCATGTC-3

 
  1. a Viral RNA quantification. b Reference gene. c Microarray confirmation and EM-induced genes, Fig. 4. d Adaptive immune responses all stages, Fig. 6. e Development of class prediction, Fig. 7.