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Table 3 Real-time qPCR primers used in the study

From: Gene expression analyses in Atlantic salmon challenged with infectious salmon anemia virus reveal differences between individuals with early, intermediate and late mortality

Genes Primers GenBank
ISAV segment 6 a 5-AGGCCAAAAACGGAAATGGA-3 AF220607
  5-CCGTCAGTGCAGTCATTGGTT-3  
18S rRNA b 5-GCCCTATCAACTTTCGATGGTAC-3 AJ427629
  5-TTTGGATGTGGTAGCCGTTTCTC-3  
Galectin-3 binding prot c 5-CCAGACCAACAGTGTTCACTTCAGC-3 BX079375
  5-ACGTGAAAGACATACCTGCCCTCAC-3  
Galectin-like 1 c 5-CAGCAACCCTTCTTCAATCCGAGA-3 CA344100
  5-TCTCCCTGTCACAGTGATGGTCTTC-3  
Tripartite motif/ring finger protein c 5-TGTTCTGTTGCTCCGTCTGTCTGGA-3 CA376536
  5-TTCAGCCAGCACGGTGTTTCTCTTC-3  
Tyr-protein kinase FRK c 5-TAGACATGGCACCATGGACCCTC-3 CA349577
  5-GGGTTCTTCAGTGCAGACAGCCA-3  
STAT1 c 5-GAACATGGAGGAGTCCAATGGAAGC-3 CA343225
  5-GGACCCTCATTTGATCTGTTGCCT-3  
CCAAT/enhancer binding protein beta c 5-TACGTCCTGGGCTATCCTGAACTGC-3 CA348284
  5-CCAGACGAACCGTTGTTGTCCA-3  
Beta-2-microglobulin c 5-TCGTTGTACTTGTGCTCATTTACAGC-3 AF180478
  5-CAGGGTATTCTTATCTCCAAAGTTGC-3  
IgM heavy chain (CH) d 5-ATACGGTGACCCTGACTTGCTACGT-3 S48652
  5-TTCTCTCCACCGGCTCATCATCA-3  
IgZ CH-like d 5-AGCACCAGGGACAAACCACCAT-3 CA372094
  5-TTCACACTCGGTGGGTTCAGAGTC-3  
IgD CH d 5-TCTTCAGGAGCTGAGGACAGATGGA-3 AF278717
  5-AAACCCATCCACCTTCCAGCTGA-3  
CD4 d 5-TGCATTGTTCCTCTCTTCCACAGC-3 AY973028
  5-CCGTCCCAAGGTACCATAGTACCAA-3  
TGF-beta d 5-AATCGGAGAGTTGCTGTGTGCGA-3 EU082211+
  5-GGGTTGTGGTGCTTATACAGAGCCA-3 AJ007836
CD8-alpha d 5-CGTCTACAGCTGTGCATCAATCAA-3 AY693391
  5-GGCTGTGGTCATTGGTGTAGTC-3  
Interferon-gamma d 5-TTCAGGAGACCCAGAAACACTAC-3 AJ841811
  5-TAATGAACTCGGACAGAGCCTTC-3  
GRB2-related adaptor protein 2 e 5-TGACTTTACTGCCACTGCTGAGGAC-3 CA353121
  5-CAGTCATCATTGGTGCCCAAGATC-3  
Annexin A1-1 e 5-CTCCAGGAAATTGAACACCGCGA-3 CA364941
  5-AAGGCTGCGATGAAGGACATGGT-3  
5-lipoxygenase activating protein e 5-TCTGAGTCATGCTGTCCGTAGTGGT-3 CA369467
  5-CCTCCCTCTCTACCTTCGTTGCAAA-3  
Regulator of G-protein signaling 1-1 e 5-GACTCCTAACCTCCAATGCTTCGAC-3 CA383094
  5-CGAATCTCTCTCCATCAGCCCATA-3  
Cytochrome P450 2K4-1 e 5-TTCACCCTCCACCCTTCACCTC-3 EV384586
  5-ATCTCAGACCCGGCTCACAGCA-3  
Galectin-9 e 5-TCGCTGATTGTGAATGGTGCTCAC-3 CX035552
  5-CAGGGTTGGAGAAGGCAATGGATT-3  
Heat shock protein HSP 90-beta-2e 5-GAACCTCTGCAAGCTCATGAAGGA-3 BX074486
  5-ACCAGCCTGTTTGACACAGTCACCT-3  
Cathepsin S e 5-CGAAGGGAGGTCTGGGAGAGGAAT-3 CA355014
  5-GCCCAGGTCATAGGTGTGCATGTC-3  
  1. a Viral RNA quantification. b Reference gene. c Microarray confirmation and EM-induced genes, Fig. 4. d Adaptive immune responses all stages, Fig. 6. e Development of class prediction, Fig. 7.