Figure 4

Immunofluorescence detection of IFN-α producing cells. Cryostat sections of colonic tissues from mice 4 hr after the administration of saline or R11567DA. Sections were multi-labeled with anti-ISG and anti-CD11b, anti-CD11c, anti-mPDCA1 or DAPI. A, ISG15 signals were observed in colonic lamina propria of R11567DA-treated mice while saline treatment produced no signal. B, C. Portions of ISG15⁺ cells (green) were co-stained (yellow) with anti-CD11b antibody (red). Nuclei were visualized by DAPI staining (blue) in Panel B. D, ISG15⁺CD11c⁺ double positive cells (yellow) were scarcely found. E, In addition to ISG15⁺CD11b⁺ double-positive cells (yellow), ISG15⁺mPDCA1⁺ double positive cells (white) were found. However, more than half of ISG15⁺ cells was stained by neither anti-CD11b nor anti-mPDCA1 antibodies (data not shown).