Table 4 Changes in gene expression of flora-reconstituted mice (quantitative realtime RT-PCR)
Gene Name* | Gene Symbol | qt realtime RT-PCR | RT-PCR | |
|---|---|---|---|---|
Fold change** | p value | primer/probe ID*** | ||
Type 1 interferon-reguratory factors | ||||
interferon regulatory factor 1 (IRF1) | Irf1 | 1.00 | - | Mm00515191_m1 |
interferon regulatory factor 3 (IRF3) | Irf3 | 1.84 | 0.003 | Mm00516779_m1 |
interferon regulatory factor 5 (IRF5) | Irf5 | 3.05 | 0.053 | Mm00496477_m1 |
interferon regulatory factor 6 (IRF6) | Irf6 | 6.47 | 0.055 | Mm00516797_m1 |
interferon regulatory factor 7 (IRF7) | Irf7 | 4.42 | <0.001 | Mm00516788_m1 |
interferon dependent positive acting transcription factor 3 gamma(IRF9) | Isgf3g | 2.87 | 0.033 | Mm00492679_m1 |
interferon consensus sequence binding protein 1(IRF8) | Irf8 | 1.89 | 0.043 | Mm00492567_m1 |
Type 1 interferon-inducible genes | ||||
interferon-induced protein with tetratricopeptide repeats 1 | Ifit1 | 4.13 | 0.029 | Mm00515153_m1 |
interferon-induced protein with tetratricopeptide repeats 2 | Ifit2 | 5.18 | 0.233 | Mm00492606_m1 |
glucocorticoid-attenuated response gene 49 (GARG-49/IRG2) | Ifit3 | 12.77 | 0.095 | Mm01704846_s1 |
guanylate nucleotide binding protein 2:mGBP-2 | Gbp2 | 3.66 | 0.069 | Mm00494575_m1 |
expressed sequence AI481100:GTPI | Iigp2 | 4.43 | 0.008 | Mm00546343_s1 |
chemokine (C-X-C motif) ligand 10:IP-10 | Cxcl10 | 14.33 | 0.133 | Mm00445235_m1 |
interferon inducible protein 1:LRG-47, Ifi1 | Irgm | 7.14 | 0.177 | Mm00492596_m1 |
lectin, galactose binding, soluble 9 | Lgals9 | 4.03 | 0.008 | Mm00495295_m1 |
interferon gamma induced GTPase | Igtp | 3.22 | 0.132 | Mm00497611_m1 |
IFN- type I-induced and dsRNA-activated kinase | Eif2ak2 | 2.07 | 0.040 | Mm00440966_m1 |
2'-5' oligoadenylate synthetase 1A | Oas1a | 3.18 | 0.046 | Mm00836412_m1 |
2'-5' oligoadenylate synthetase 1B | Oas1b | 13.05 | 0.052 | Mm00449297_m1 |
TAP binding protein | Tapbp | 1.86 | 0.046 | Mm00493417_m1 |
Type I interferon receptors | ||||
interferon (alpha and beta) receptor 1 | Ifnar1 | 1.84 | 0.017 | Mm00439544_m1 |
interferon (alpha and beta) receptor 2 | Ifnar2 | 2.06 | 0.010 | Mm00494916_m1 |
Complement-related genes | ||||
complement component 1, q subcomponent, alpha polypeptide | C1qa | 1.87 | 0.178 | Mm00432142_m1 |
complement component 3 | C3 | 2.08 | 0.141 | Mm00437858_m1 |
serine (or cysteine) proteinase inhibitor, clade G, member 1: complement 1 inhibitor | Serping1 | 2.18 | 0.005 | Mm00437834_m1 |
MHC-related genes | ||||
Histocompatibility 2, class II antigen A, alpha | H2-Aa | 2.72 | 0.266 | Mm01609331_m1 |
MHC class II H2-I-A-beta gene (k haplotype) | H2-AB1 | 3.42 | 0.041 | H2-AB1-TG04 |
MHC (A.CA/J(H-2K-f) class I antigen | H2-Q1 | 3.47 | 0.356 | Mm00657093_g1 |
MHC class I Q4 beta-2-microglobulin (Qb-1) gene | H2-Q4 | 3.83 | 0.032 | H2-Q4-TG09 |
Q8/9d gene | LOC386462 | 11.69 | 0.153 | H2-Q78-TG08 |
Histocompatibility 2, T region locus 10+17 | H2-T10/22/9 | 8.06 | 0.006 | H2-T1017-TG11 |
Histocompatibility 2, T region locus 23 | H2-T23 | 1.10 | 0.762 | Mm00439246_g1 |
Other molecules | ||||
cytotoxic T lymphocyte-associated protein 2 alpha | Ctla2a | 9.48 | 0.139 | Mm00484032_g1 |
CD52 antigen | Cd52 | 3.05 | 0.095 | Mm00489055_m1 |
signal transducer and activator of transcription 1 | Stat1 | 4.66 | 0.026 | Mm00439518_m1 |
signal transducer and activator of transcription 2 | Stat2 | 3.81 | 0.015 | Mm00490880_m1 |