Figure 10

Effects of ERK1 and ERK2 knockdown affect developmental signaling pathways confirmed by whole mount in situ hybridization. The zebrafish embryos were injected with 3.4 ng ERK1MO (B,E,H,K,N,Q) or ERK2MO (C,F,I,L,O,R) and in situ expression patterns, were compared to wild type embryos (A,D,G,J,M,P). The embryos were fixed at 4.5hpf, processed for whole mount in situ hybridization, and imaged (animal pole view, dorsal to right). A,B,C: goosecoid (gsc, presumptive shield/dorsal organizer); D,E,F: lft1/antivin, (blastula margin); G,H,I: vox (expressed in blastula, but the dorsal most region); J,H,L vent (ventral blastula margin); M,N,O, notail (ntl, blastula margin); P,Q,R,: tbx6 (margin)