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Table 2 Quantitative RT-PCR validation of 8 selected genes.

From: Transcriptome analysis identifies pathways associated with enhanced maternal performance in QSi5 mice

Gene Symbol

Microarrays

Real-time PCR

 

FC

P-value

FC

P-value

Kif5b

12.17

< 0.001

3.14

0.005

Ptger2

4.58

< 0.001

3.42

0.01

Dkkl1

-3.76

< 0.001

-10.01

0.006

Grb10

-2.68

< 0.001

-10.32

0.09

Tnc

-1.76

0.001

-4.16

0.06

Wnt4

1.13

0.229

1.82

0.047

cyclin D1

1.08

0.66

1.95

0.12

cmyc

0.85

0.08

1.02

0.97

  1. Comparison between array experiment and real-time PCR for 8 selected genes is shown. Log-fold change in real-time PCR was calculated from the difference in normalised expression between the CBA and QSi5 strains of mice. P-value was calculated from 2-tailed Student's t-test.
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BMC Genomics

ISSN: 1471-2164

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