Figure 2

Gene expression in different cell lines. Levels of expression of ADAM23, FAP, GPNMB and PRSS3 were evaluated in fibroblasts (HuFb and Malme3) and smooth muscle cells (USMAC) as well as in tumor cell lines (1A9, SKOV3, HT29 and MDA-MB-231). Gene expression of each target gene was normalized to 18s rRNA for each cell type (ΔCt = Ct_target - Ct_18s). Average of ΔCt of the target gene from tumor derived ECs (Figure 1) was assumed as reference (ΔCt_ref). Fold differences for each cell type were calculated according to the comparative ΔΔCt method (Fold difference = 2^{-(ΔCt each celltype-ΔCt ref)}) and expressed as percentage relative to tumor-derived EC (100%).