Figure 1

Biallelic expression of IGF2 in M. domestica is dependent on CpG methylation. A-C) RNA fluorescence in situ hybridization (FISH) detecting nascent transcripts of IGF2 (red) and DNA FISH detecting DNA copies of IGF2 (green) to interphase cells (counterstained with dapi, black and white). Individual probe images for dapi, DNA FISH, RNA FISH and the merge image are shown from left to right. A) FISH on control (no treatment) cells showing no IGF2 expression. B) FISH on control (no treatment) cells showing monoallelic IGF2 expression (RNA transcript is indicated with an arrowhead). C) FISH on 5-azacytidine treated cells showing biallelic expression (RNA transcripts are indicated with an arrowhead). D) Total cell counts/well of 0, 1 or 2 RNA FISH signals in experiment and control micrographs as designated.