Figure 4From: Circadian genomics of the chick pineal gland in vitroMicroarray validation. qPCR was used to validate rhythmic expression of the following genes under LD conditions: TrH, HIOMT, cry1, per3, cystatin c, NF1X, and purpurin (A-G, respectively). Cosinor functions fitted to data from microarray analysis using GeneSpring output (black lines) and from qPCR analysis (grey lines) are plotted. Cosinor analysis and ANOVA were performed on each data set. White bars indicate lights on, and black bars indicate lights off. Light hatched bars indicate subjective day, while dark hatched bars indicate subjective night.Back to article page