LCMD of villus epithelial and Paneth cells. A. Villus and crypt epithelial cells were excised from sections of formalin fixed human ileal tissue samples by LCMD. Areas where cells from each lineage were captured are labeled. The mRNA from the isolated cells was amplified, labeled and analyzed using both microarray and RT-PCR based methodologies. B. Schematic diagram of the cell types that comprise the human ileal epithelium. Epithelial cell lineages, including adsorptive, enteroendocrine, goblet, and Paneth cells are color-coded and their distribution within the epithelium is shown. The location of stem cells, shown in green, and the bidirectional cell migration is depicted in the magnified crypt region on the right.