Figure 5

Quantitative real-time PCR analysis of selected mRNAs in pools of villus and crypt epithelial cells. Real-time fluorescence monitoring of PCR reactions using cDNA from villus and crypt samples obtained by LCMD. PCR primer pairs for HD5, Reg3A, aldolase-B or β-Actin were used in PCR reactions with cDNA templates from villus epithelial cells (VEC) or crypt epithelial cells (CEC). NTC, no template control. The cDNA was used as a template in real-time PCR analysis with specific primers (Table 1).