Figure 1From: Chloroplast genome sequencing analysis of Heterosigma akashiwo CCMP452 (West Atlantic) and NIES293 (West Pacific) strainsFosmid cloning technique. High molecular weight, total DNA is subject to pulse-field electrophoresis to recover sheared DNA of 45 to 50 kb. This DNA is used to generate a fosmid library which is selectively screened for cpDNA-containing clones, which are then sequenced, annotated and assembled.Back to article page