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Table 3 loj genetically interacts with Drosophila NF-κB genes Dif and Rel

From: Loss of p24 function in Drosophila melanogaster causes a stress response and increased levels of NF-κB-regulated gene products

Genotype # eclosed % expected eclosing
Dif 2 /+; loj 00898 /+ 369 100%
Dif 2 ; loj 00898 /+ 192 52.0%
Dif 2 /+; loj 00898 122 33.1%
Dif 2 ; loj 00898 18 4.9%
Dif 2 /+; loj/+ 94 100%
Dif 2 ; loj/+ 47 50.0%
Dif 2 /+; loj 00898 /loj 04026 55 58.5%
Dif 2 ; loj 00898 /loj 04026 14 14.8%
dl 1 cn 1 sca 1 /+; loj 00898 /+ 94 100%
dl 1 cn 1 sca 1 ; loj 00898 /+ 10 10.6%
dl 1 cn 1 sca 1 /+; loj 00898 13 13.8%
dl 1 cn 1 sca 1 ; loj 00898 11 11.7%
loj00898, RelE20/+ 315 100%
loj00898, RelE20 6 1.9%
loj00898, RelE20 or loj00898/+ 135 100%
loj00898, RelE20/loj00898 71 100.5%
loj00898, RelE20 or RelE20/+ 57 100%
loj00898, RelE20/RelE20 20 70.0%
imd EY08573 /+; loj 00898 /+ 111 100%
imd EY08573 ; loj 00898 /+ 60 54.0%
imd EY08573 /+; loj 00898 17 15.3%
imd EY08573 ; loj 00898 17 15.3%
  1. For tests with Dif, dl, and imd each of the four expected progeny classes should be represented at equal frequency (25% each). Only two types of progeny are produced in the loj00898, RelE 20/TM3, Sb cross, so each class should account for 50% of the offspring. Homozygous loj00898, loj04026, Dif2 or dl1 mutations independently affect adult viability, while RelE 20and imdEY 08573stocks are maintained as homozygotes. Adult eclosion is dramatically reduced when Dif2 or RelE 20(but not dl1 or imdEY 08573) homozygous mutations are introduced into the loj00898 homozygous mutant background. Similar results were obtained for 5 independent loj00898, RelE 20recombinant strains and different genetic backgrounds containing imdEY 08573. Large numbers of loj00898, RelE 20/RelE 20and loj00898, RelE 20/loj00898 (or loj04026) survive to adulthood (70–100% of expected), indicating that the decreased eclosion of loj00898, RelE 20animals is largely due to effects from mutations in both genes. Although homozygous mutations in loj or dl decrease adult viability, we did not observe additional, large effects when both genes are mutated in 2 different genetic backgrounds.