Loss of p24 function in Drosophila melanogaster causes a stress response and increased levels of NF-κB-regulated gene products

Table 3 loj genetically interacts with Drosophila NF-κB genes Dif and Rel

For tests with Dif, dl, and imd each of the four expected progeny classes should be represented at equal frequency (25% each). Only two types of progeny are produced in the loj⁰⁰⁸⁹⁸, Rel^{E 20}/TM3, Sb cross, so each class should account for 50% of the offspring. Homozygous loj⁰⁰⁸⁹⁸, loj⁰⁴⁰²⁶, Dif² or dl¹ mutations independently affect adult viability, while Rel^{E 20}and imd^{EY 08573}stocks are maintained as homozygotes. Adult eclosion is dramatically reduced when Dif² or Rel^{E 20}(but not dl¹ or imd^{EY 08573}) homozygous mutations are introduced into the loj⁰⁰⁸⁹⁸ homozygous mutant background. Similar results were obtained for 5 independent loj⁰⁰⁸⁹⁸, Rel^{E 20}recombinant strains and different genetic backgrounds containing imd^{EY 08573}. Large numbers of loj⁰⁰⁸⁹⁸, Rel^{E 20}/Rel^{E 20}and loj⁰⁰⁸⁹⁸, Rel^{E 20}/loj⁰⁰⁸⁹⁸ (or loj⁰⁴⁰²⁶) survive to adulthood (70–100% of expected), indicating that the decreased eclosion of loj⁰⁰⁸⁹⁸, Rel^{E 20}animals is largely due to effects from mutations in both genes. Although homozygous mutations in loj or dl decrease adult viability, we did not observe additional, large effects when both genes are mutated in 2 different genetic backgrounds.