Figure 1

Features of the R&R Consensus in An. gambiae and their relationship to the pfam00379 motif. The longest region that generally could be aligned across An. gambiae CPRs is shown beginning with a proline or glycine two or three sites prior to the aromatic triad and ending with a proline or glycine eight positions C-terminal to the final invariant glycine. Our alignment of this region was 65 positions long for aligned RR-2 genes, but the complete alignment was 83 positions long due to length variation in RR-1 genes. The pfam00379 alignment includes seven additional positions N-terminal to our alignment. The region used in the phylogeny extended further in the N-terminal and C-terminal directions as described in the text. The shaded region on the top line was double-weighted because this encompasses the principal features of the R&R Consensus that are present in virtually all An. gambiae CPRs.