Figure 1
From: Improved microarray gene expression profiling of virus-infected cells after removal of viral RNA
Coronavirus RNA replication strategy. MHV-A59 produces a nested set of subgenomic mRNAs (sgRNAs) that all have identical 5' and 3' terminal ends. Via discontinuous transcription, during the synthesis of the minus strand, sgRNAs obtain the 5' leader sequence fused to a transcriptional regulating sequence (TRS, indicated by the numbered small black boxes), followed by a protein-coding region that is flanked at the 3' terminus by an untranslated region (3'UTR) and a polyA-tail (indicated as AA(A)n). Note that the nucleocapsid (N) gene is present in all sgRNAs. The three biotinylated oligonucleotides, which are used in the vRNA removal procedure and which are complementary to three regions in the viral genome, are indicated by the underscores and the letters A, B, and C (see Table 1 for the nucleotide sequences).